The psoas RCSA and multifidus FI can be used as predictive factors for functional performance in LSS patients.

Candida albicans is responsible for a number of life-threatening infections and causes considerable morbidity and mortality in immunocompromised patients. Previous studies of C. albicans pathogenesis have suggested several steps must occur before virulent infection, including early adhesion, invasion, and late tissue damage. However, the mechanism that triggers C. albicans transformation from yeast to hyphae form during infection has yet to be fully elucidated. This study used a systems biology approach to investigate C. albicans infection in zebrafish. The surviving fish were sampled at different post-infection time points to obtain time-lapsed, genome-wide transcriptomic data from both organisms, which were accompanied with in sync histological analyses. Principal component analysis (PCA) was used to analyze the dynamic gene expression profiles of significant variations in both C. albicans and zebrafish. The results categorized C. albicans infection into three progressing phases: adhesion, invasion, and damage. Such findings were highly supported by the corresponding histological analysis. Furthermore, the dynamic interspecies transcript profiling revealed that C. albicans activated its filamentous formation during invasion and the iron scavenging functions during the damage phases, whereas zebrafish ceased its iron homeostasis function following massive hemorrhage during the later stages of infection. Most of the immune related genes were expressed as the infection progressed from invasion to the damage phase. Such global, inter-species evidence of virulence-immune and iron competition dynamics during C. albicans infection could be crucial in understanding control fungal pathogenesis.

Highly efficient twisted nematic liquid crystal polarization gratings achieved by microrubbing Appl. Phys. Lett. 101, 041107 (2012) A polarization-independent liquid crystal phase modulation using polymer-network liquid crystals in a 90° twisted cell J. Appl. Phys. 112, 024505 (2012) Fast switchable grating based on orthogonal photo alignments of ferroelectric liquid crystals This work proposes two mechanisms for switching the direction of stripes in cholesteric liquid crystal (CLC) gratings. The stripe direction depends on the ratio of cell gap to the natural pitch length (d/P 0 ) of a CLC sample. Electrical switching is based on the different pitches at the planar and the transient planar states. Optical switching, however, changes pitch by using the photo-isomerization effect of the azobenzene doped in a CLC sample. Using the two mechanisms, we can switch the stripe directions in two orthogonal directions. Furthermore, the beam-steering capability of CLC gratings also remains effective after switching directions. V C 2012 American Institute of Physics. [http://dx.

BackgroundSignal transduction is the major mechanism through which cells transmit external stimuli to evoke intracellular biochemical responses. Diverse cellular stimuli create a wide variety of transcription factor activities through signal transduction pathways, resulting in different gene expression patterns. Understanding the relationship between external stimuli and the corresponding cellular responses, as well as the subsequent effects on downstream genes, is a major challenge in systems biology. Thus, a systematic approach is needed to integrate experimental data and theoretical hypotheses to identify the physiological consequences of environmental stimuli.ResultsWe proposed a systematic approach that combines forward and reverse engineering to link the signal transduction cascade with the gene responses. To demonstrate the feasibility of our strategy, we focused on linking the NF-κB signaling pathway with the inflammatory gene regulatory responses because NF-κB has long been recognized to play a crucial role in inflammation. We first utilized forward engineering (Hybrid Functional Petri Nets) to construct the NF-κB signaling pathway and reverse engineering (Network Components Analysis) to build a gene regulatory network (GRN). Then, we demonstrated that the corresponding IKK profiles can be identified in the GRN and are consistent with the experimental validation of the IKK kinase assay. We found that the time-lapse gene expression of several cytokines and chemokines (TNF-α, IL-1, IL-6, CXCL1, CXCL2 and CCL3) is concordant with the NF-κB activity profile, and these genes have stronger influence strength within the GRN. Such regulatory effects have highlighted the crucial roles of NF-κB signaling in the acute inflammatory response and enhance our understanding of the systemic inflammatory response syndrome.ConclusionWe successfully identified and distinguished the corresponding signaling profiles among three microarray datasets with different stimuli strengths. In our model, the crucial genes of the NF-κB regulatory network were also identified to reflect the biological consequences of inflammation. With the experimental validation, our strategy is thus an effective solution to decipher cross-talk effects when attempting to integrate new kinetic parameters from other signal transduction pathways. The strategy also provides new insight for systems biology modeling to link any signal transduction pathways with the responses of downstream genes of interest.

Abstract. In this paper, we study a discrete diffusive equation with a bistable nonlinearity. For this equation, there are three types of traveling fronts. By constructing some suitable pairs of super-sub-solutions, we show that there are only two types of entire solutions originating from three fronts of this equation. These results show us some new dynamics of this discrete diffusive equation.