Studies have indicated that trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well-known traditional Chinese medicinal plant), produces antitumor effects on various types of cancer cells. However, the effects of TCS on glioma cells are poorly understood. The objective of this study was to investigate the antitumor effects of TCS on the U87 and U251 cell lines. The in vitro effects of TCS on these two cell lines were determined using a Cell Counting Kit-8 (CCK-8) assay, Annexin V-FITC staining, DAPI staining, Transwell assays, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacar-bocyanine iodide (JC-1) staining and western blotting, which was utilized to assess the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) and key proteins in the Wnt/β-catenin signaling pathway. Our data indicated that TCS inhibited the proliferation of glioma cells in a dose- and time-dependent manner and played a role in inhibiting glioma cell invasion and migration. Additional investigation revealed that the expression levels of LGR5 and of key proteins in the Wnt/β-catenin signaling pathway were markedly decreased after TCS treatment. The results suggest that TCS may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/β-catenin signaling pathway. In the future, in vivo experiments should be conducted to examine the potential use of this compound as a novel therapeutic agent for gliomas.

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<b><i>Background:</i></b> Focal cortical dysplasia (FCD) is one of the most important pathogenic findings in patients with extratemporal lobe epilepsy. Magnetic resonance imaging (MRI)-negative is the most important negative factor to predict postoperative seizure freedom; however, FCD-I and part of FCD-IIa are MRI-negative on routine MRI. <b><i>Objectives:</i></b> To explore the diagnostic values of 7T MRI and its new scan sequences in epilepsy patients with FCD-IIa. <b><i>Methods:</i></b> To include patients with focal seizure and suspicious focal abnormal imaging on 3T MRI during preoperative evaluation and perform a 7T MRI scan with white matter-suppressed (WMS) and gray-white matter tissue border enhancement (GWBE) sequences, resective epilepsy surgery, and postoperative pathological finding of FCD-IIa. The preoperative qualitative and localization significance of 7T MRI and 3T MRI in lesions with FCD-IIa was compared, and then, the imaging characteristics of lesions with FCD-IIa on 7T MRI were analyzed. <b><i>Results:</i></b> Ten cases were enrolled in this study. Seven tesla MRI presented high spatial resolutions and a high signal-to-noise ratio. WMS and GWBE could selectively suppress the signal of special tissue and improved the possibility of FCD findings. FCD-IIa showed abnormal thickness of gray matter and a blurring border and was hypointense on 7T MRI compared with 3T MRI. Seven patients showed improvement in the qualitative diagnosis strength grade of FCD, and 6 subjects showed improvement in the localization strength grade of the lesion border after careful reading of the 7T MR images. Significant differences were found in the qualitative diagnosis of FCD (<i>p</i> &#x3c; 0.05) and localization of the lesion border (<i>p</i> &#x3c; 0.05) between the neuroimaging diagnoses based on 3T MRI and the findings based on 7T MRI. <b><i>Conclusion:</i></b> 7T MRI with WMS and GWBE sequences shows application value in the preoperative imaging diagnosis of lesions with FCD-IIa in epilepsy patients.

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