Correlation of triglyceride (TG)-to-high density lipoprotein cholesterol (HDL-C) ratio (TG/HDL-C) and the survival of gastric cancer (GC) remain unclear. The purpose of this study was to explore the precise effect of preoperative TG/HDL-C on clinical outcomes in GC patients. Patients with GC were enrolled from 2006 to 2014. A total of 957 individuals from a single center were divided into prospective training and retrospective test cohorts. The optimal cutoff value of TG/HDL-C was determined using X-tile software to separate the training cohort into low and high survival groups according to TG/HDL-C levels. Survival analyses were performed using Kaplan-Meier curves and a Cox proportional hazards regression model. Preoperative TG/HDL-C and clinical outcomes were obtained to determine the prognostic significance of serum lipids in the training and test cohorts. We observed that high TG and TG/HDL-C were significantly correlated with poor outcome in GC patients, and high TG/HDL-C harbored the highest area under curve to independently predict 5-year overall survival in two cohorts. Furthermore, c-index of the prognostic nomogram including TG/HDL-C was significantly higher than that without it. In summary, TG/HDL-C was an efficient and independent prognostic factor to predict 5-year case fatality of GC patients and to improve the efficacy of its prognostic nomogram.

ZLN and YSW are contributed equally to this work.

No abstract

Background Paraoxonases (PONs) are a family of orphan enzymes with multiple functions, including anti-inflammatory, antioxidative, antiatherogenic activities. Studies have suggested that genetic variations in PON1 and PON2 are associated with ischemic stroke (IS) risk; however, the conclusion remains unclear in the Chinese population. Methods To investigate the susceptibility of genetic variations in PON1 and PON2 to risk of IS and its subtypes, this case–control study was carried out on a Chinese population comprising 300 IS patients and 300 healthy controls. Genotypes of six genetic variations in PON1 and PON2 were identified with an improved multiplex ligase detection–reaction technique. Results PON1 rs662 was associated with increased risk of IS (CT vs. TT — OR adjusted 1.79, 95% CI 1.08–2.97; p =0.025). Stratified analysis for patients by sex revealed that the significant association of PON1 rs662 with IS risk was maintained in the male cohort (CT vs. TT — OR adjusted 2.59, 95% CI 1.29–5.21 [ p =0.009]; CT/CC vs. TT — OR adjusted 2.03, 95% CI 1.05–3.93 [ p =0.036]), but not in the female cohort. Analysis according to IS subtype revealed that PON1 rs662 genetic variation was an increased risk in the subcohort of patients with large-artery atherosclerosis (CT/CC vs. TT — OR adjusted 2.31, 95% CI 1.09–4.91; p =0.029), but not in patients with other types of IS. Conclusion This study suggested that PON1 rs662 presented a potential risk of IS, especially for males, and this association was more obvious for large-artery atherosclerosis.

Background Inflammation proteins play an important role in stroke occurrence. IL1A, IL1B, PTGS2, MMP2 , and MMP9 were the mediators involved in the immune response, and the association of these genetic variations with ischemic stroke (IS) risk was still unclear. Methods To investigate the susceptibility of genetic variations of IL1A, IL1B, PTGS2, MMP2 , and MMP9 to IS risk, we performed a case–control study involving 299 patients and 300 controls in a Chinese population. Thirteen genetic variations of investigated genes of all participants were genotyped using an improved multiplex ligase detection–reaction technique. Results No SNP in all genes showed an association with overall IS. However, in subgroup analysis, PTGS2 rs689466 (dominant model: CT vs TT – OR adjusted = 2.51, 95% CI: 1.22–5.16, p = 0.012; co-dominant model: CT/CC vs TT – OR adjusted = 2.53, 95% CI: 1.26–5.07, p = 0.009; additive model – OR adjusted = 2.26, 95% CI: 1.19–4.28, p = 0.013) and rs5275 (dominant model: GG vs AA – OR adjusted = 0.31, 95% CI: 0.12–0.80, p = 0.016; co-dominant model: GA/GG vs AA – OR adjusted = 0.45, 95% CI: 0.21–0.95, p = 0.036; additive model – OR adjusted = 0.60, 95% CI: 0.39–0.92, p = 0.020) were associated with IS type of small-vessel occlusion. Conclusion Our study suggested that PTGS2 rs689466 C and rs5275 A were potentially associated with IS subtype of small-vessel occlusion. Our result should be confirmed with further large sample sized studies.