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The use of a novel procedure of solid-phase extraction with molecularly imprinted polymers (MISPE) has been described. A MISPE procedure relying on tailor-made, artificial-mimic materials capable of selectively rebinding target analytes (steroids) based on a combination of recognition mechanisms, such as size, shape and functionality, was custom designed for progesterone and testosterone. The combination of MISPE with LC/DAD is a simple and an efficient method for the determination and quality control of progesterone and testosterone in human urine samples.Keywords Steroids · Molecularly imprinted adsorbents · MISPE · HPLC · DAD Abbreviations AIBN 2,2 -azobis(2-methylpropionitrile) α separation factor BME benzyl methyl ether DAD diode array detector EDMA ethylene glycol dimethacrylate HPLC high performance liquid chromatography k retention factor LOQ limit of quantification LOD limit of detection MAA methacrylic acid MIP molecularly imprinted polymer

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