Molecularly imprinted adsorbents for preconcentration and isolation of progesterone and testosterone by solid phase extraction combined with HPLC

Ričanyová, Júlia; Gadzała‐Kopciuch, Renata; Reiffová, Katarína; Bazeľ, Yaroslav; Buszewski, Bogusław

doi:10.1007/s10450-010-9265-7

Cited by 34 publications

(21 citation statements)

References 36 publications

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“…The resulting molecularly imprinted polymers (MIPs), which are stable, simple to prepare, reusable, and highly selective, have been applied as SPE sorbents in many fields, including biology (Ričanyová et al 2010), environmental studies (Núñez et al 2010), pharmaceuticals (Zhang et al 2009), and food analysis (Theodoridis et al 2006). However, traditional MIPs can only specifically recognize the template molecule and have low adsorption for other substances.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography

Wang

Zhang

et al. 2015

Food Anal. Methods

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In this study, a molecularly imprinted polymer that can selectively recognize dimethoate, isocarbophos, and methyl parathion was synthesized. Using this compound as sorbent, a new method for simultaneous determination of the three organophosphorus pesticides by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography was established. The factors affecting the preconcentration and sensitivity were studied in detail. Under optimal conditions, the limits of detection of this method for dimethoate, isocarbophos, and methyl parathion were 19.78, 8.73, and 17.41 μg/kg, respectively. The relative standard deviation for five replicates of 0.01 mg/L-mixed solutions was in the range of 1.8-4.2 %. Cucumber sample spiked with the three organophosphorus pesticides at levels of 0.125 and 0.250 μg/g were examined using this method, good recoveries were obtained ranging from 82.5 to 95.6 %. Moreover, this method was also successfully used for the detection of the three organophosphorus pesticides in cauliflower sample.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography

Wang

Zhang

et al. 2015

Food Anal. Methods

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“…As can be seen, the present method had a relatively low LOD among the other reported approaches. 9,10,23,24 Meanwhile, Fe 3 O 4 @TSTO-MIPs used as SPE extractant possessed satisfactory selectivity and excellent stability, compared with the standard SPE C18 sorbents 24 and immunoaffinity capillary column, 10 respectively. In conclusion, the present method is reliable, effective, and sensitive.…”

Section: Methods Validation and Real Sample Analysismentioning

confidence: 99%

Synthesis of magnetic molecularly imprinted polymers with excellent biocompatibility for the selective separation and inhibition of testosterone in prostate cancer cells

Tang¹,

Li²,

Jia³

et al. 2017

IJN

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Purpose Androgen plays an important role in the progression of prostate cancer. In the present study, novel magnetic molecularly imprinted polymers (MMIPs) with good biocompatibility were produced for the selective separation and inhibition of testosterone in prostate cancer cells. Materials and methods MMIPs were prepared by using magnetic nanospheres, gelatin, and testosterone as the supporting materials, functional monomer, and the template molecule, respectively. The characterization of the resultant products was investigated by transmission electron microscopy, X-ray diffraction, and vibrating sample magnetometry. To test whether MMIPs can remove testosterone in biologic samples, human LNCaP (androgen-dependent) and C4-2 (androgen-independent) prostate cancer cells were selected as cell models. The translocation of androgen receptor (AR) was detected by immunofluorescence assay, and the expression of PSA mRNA was detected by real-time quantitative polymerase chain reaction analysis. Cell flow cytometry analysis was performed to detect cell cycle arrest. Results The synthesized nanomaterials (MMIPs) possessed high crystallinity, satisfactory superparamagnetic properties, and uniform imprinted shell, and exhibited high adsorption capacity, fast kinetics, and high selectivity for testosterone. Moreover, the obtained imprinted nanomaterials could selectively enrich and detect testosterone in the LNCaP cell samples as a solid-phase extractant coupled with high-performance liquid chromatography. In addition, the MMIPs could freely enter prostate cancer cells and suppress the translocation of AR into the cell nucleus. We further found that MMIPs inhibited upregulation of AR downstream target genes in LNCaP and C4-2 cells; also, MMIPs inhibited cell growth and induced obvious cell cycle arrest in androgen-dependent LNCaP cells, but had no obvious effect on androgen-independent C4-2 cells. Conclusion Our results indicate that the obtained imprinted nanomaterials can specifically and effectively bind testosterone and recover it from prostate cancer cells. Moreover, the MMIPs can freely enter prostate cancer cells and block the activation of testosterone-AR pathway. Thus, the MMIPs may be a new option for antiandrogen therapy in prostate cancer.

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“…These materials are capable of specifically recognizing and binding the template molecule, from a mixture of structurally related compounds. Molecularly imprinted materials are used in a large variety of applications, such as biosensor assays; stationary phases for chromatography, as well as in organic compounds separations from complex mixtures …”

Section: Introductionmentioning

confidence: 99%

Increasing the adsorption capacity and selectivity of poly(vinyl alcohol) hydrogels by an alternative imprinting technique

Paţachia

Croitoru

2015

J of Applied Polymer Sci

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In this work, we report an innovative alternative imprinting method for obtaining chemically crosslinked poly(vinyl alcohol) hydrogels with b-cyclodextrin as template. The materials present high affinity toward the template that imprinted them, revealed by the higher sorption yield of b-cyclodextrin and higher selectivity factors of the imprinted material, by comparing with the nonimprinted reference. The imprinting kinetic and mechanism has been demonstrated by adsorption studies, binding isotherms and Scatchard analysis and is in good correlation with the information regarding the morphology of the materials, determined by fluorescence microscopy and atomic force microscopy. By using a novel fluorescence spectroscopy method of the starting polymer solution, the optimum amount of imprinting template could be determined.

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Molecularly imprinted adsorbents for preconcentration and isolation of progesterone and testosterone by solid phase extraction combined with HPLC

Cited by 34 publications

References 36 publications

Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography

Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography

Synthesis of magnetic molecularly imprinted polymers with excellent biocompatibility for the selective separation and inhibition of testosterone in prostate cancer cells

Increasing the adsorption capacity and selectivity of poly(vinyl alcohol) hydrogels by an alternative imprinting technique

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