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Antioxidant assay was performed to count the total phenolic content in coelomic fluid of Eisenia fetida. The worms were cultured in lab until they became sexually mature. The study focused on total phenolic compound determination using two solvents in a single species of earthworm. It was analysed that the extraction of phenolic compound with 85% ethanol yielded high phenol content, 208.6 mg GAE\L Eisenia fetida. Extraction with 85% methanol on the other hand, yielded 189.1 mg GAE\L. Eisenia fetida ethanolic extract in the sample manifested high total phenolic content than methanolic extract. Phenols are more readily soluble in ethanol and safe for human consumption. Methanol is used as solvent for low molecular weight polyphenols. The study concludes that ethanol extract of Eisenia fetida coelomic fluid contains significant amount of phenols. Phenols have an antioxidant effect and can be used as natural antioxidants for curing ailments related with inflammation and oxidative stress. This study will help in bringing attention towards the therapeutic utility of earthworms.

Nepal has an abundance of biomass resources. Heavy dependence on traditional source and waste management resulting from agricultural waste is one of the major problem in our country. Briquetting from agricultural waste can be an option for resource management. This study was undertaken to investigate the physical and combustion properties of briquettes produced from dried banana tree waste (leaves + pseudostem). Altogether four types of briquettes were produced, two from uncharred biomass using screw extruder and hydraulic jack briquetting machine and other two briquettes were produced from uncharred biomass/charcoal using screw extruder and beehive briquetting machine.For charred briquette, the density of screw extruder briquette (1.06 g/cm3) was higher than hydraulic briquette (0.33 g/cm3). For uncharred briquette, the density was higher (0.72 g/cm3) for screw extruder than beehive briquette (0.44 g/cm3). The Moisture content of briquettes varied from 7.23 to 11.04%, Volatile matter varied from 11 to 68.74%, Ash content varied from 4.94 to 49.43% and fixed carbon content varied from 15.27 to 32.34%. The calorific values of the briquettes ranged from 2462.0827 - 3899.193 kcal/kg, while the thermal efficiency ranges between 24.02– 30.71%, other results shows that the average burning rate between 3.55 and 6.41 g/min. Screw extruder briquette from uncharred Banana tree waste and beehive from charred/carbonized banana tree waste biomass was found to be comparatively better from the present study.