Feline infectious peritonitis (FIP) cats show a decrease in peripheral blood lymphocyte counts, and a particularly marked decrease in T cells including CD4+ and CD8+ cells. In this study, we showed that lymphopenia observed in FIP cats was due to apoptosis, and that the ascitic fluid, plasma, and culture supernatant of peritoneal exudate cells (adherent cells with macrophage morphology, or PEC) from FIP cats readily induced apoptosis in specific pathogen-free cat peripheral blood mononuclear cells, particularly CD8+ cells. In addition, TNF-alpha released from macrophages and TNF-receptor (TNFR) 1 and TNFR2 mRNA expression in lymphocytes were closely involved in this apoptosis induction. In particular, in CD8+ cells cultured in the presence of the PEC culture supernatant, the expression levels of TNFR1 and TNFR2 mRNA were increased, indicating that CD8+ cells are more susceptible to apoptosis induction by TNF-alpha than other lymphocyte subsets, particularly B cells (CD21+ cells). The results of this study suggest that TNF-alpha, produced by virus-infected macrophages, is responsible for induction of apoptosis in uninfected T cells, primarily CD8+ T cells.

Human T-cell lymphotropic virus type 1 (HTLV-1) induces adult T cell leukemia/lymphoma (ATLL), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and carrier. Approximately half of ATLL patients have direct skin involvement of neoplastic cells. However, there exist HTLV-1-associated reactive eruptions with a predominant infiltrate of non-neoplastic CD8+ T cells in ATLL, HAM/TSP and carrier. A 50-year-old Japanese female HTLV-1 carrier had several episodes of itchy, indurated erythema that occurred diffusely on the face and neck, lasted for 2 weeks and spontaneously subsided without sequelae. Histopathologically, CD3+ T cells infiltrated the upper dermis, and part of the infiltrating cells were CD4+CD25+, sharing the phenotype with ATLL neoplastic cells. An aggregate of CD8+ T cells bearing the cytotoxic molecule TIA-1 was also present. It is possible that skin-affinitive HTLV-1+CD4+ T cells propagated and subsequently disappeared as a result of cytotoxic T cell attack.

Abstract:This study aims to applicate the Biometric impression trays for Japanese edentulous patients.

[ Oral Med Pathol 2007; 12: 11-17]

NAGAYAMA Motohiko 3 ) ・ TANUMA Jun-ichi 3 ) ・ SUMITOMO Shinichiro 2 ): Glandular odontogenic cysts are odontogenic developmental cysts that commonly arise in the mandible. Their frequency of onset in the maxilla is low. We report a glandular odontogenic cyst arising in the maxillary premolar region. The patient was a 36-year-old man who was examined at our department because of swelling of the gum as the main complaint. At the first examination, swelling with pulsation was observed in the maxillary canine and first premolar gum regions. However, the maxillary canine and first premolar showed vital reactions when an electric pulp tester was used. Computed tomography revealed bone absorption with buccal cortical bone loss in a well-defined area, 10 mm in diameter, including the maxillary canine and first premolar regions. However, there was no communication between the region of bone loss and the apex. As a maxillary bone cyst was suspected, cystectomy was performed with the patient under local anesthesia. During surgery, detachment of the cyst was achieved easily, and after the excision, the surrounding bone surface was smoothened. A hemostatic splint was placed on the open wound. The patient was given a diagnosis of a glandular odontogenic cyst based on the histopathological findings of the excised specimen. The patient' s progress continues to be positive, with no recurrence as of 2 years 3 months after surgery. : glandular odontogenic cyst (腺性歯原性囊胞) ，premolar region of the maxilla (上顎小臼歯部) ， odontogenic cyst(歯原性囊胞) 1) 市立島田市民病院歯科口腔外科 (主任：田中四郎部長) 2) 朝日大学歯学部口腔病態医療学講座口腔外科学分野 (主任：住友伸一郎教授) 3) 朝日大学歯学部口腔病態医療学講座口腔病理学分野 (主任：田沼順一教授) 1)