A highly potent attractant of zoospores of Aphanomyces cochlioides, a causal fungus of the root rot disease of spinach (Spinaeia oleracea), was isolated from spinach roots, and its structure was determined by spectroscopic evidence and chemical synthesis as cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone, 1). A chromosorb particle prepared by soaking in solution of 1 showed a potent attracting activity toward the zoospores using concentrations of 1 above 10 -9 or 10 -10 M.

Gas-liquid chromatography (GLC) analysis revealed a high content of very long chain fatty acids in the marine sponge Halichondria panicea from the coast of south Hokkaido, Japan. Three major components of the fatty acids were concentrated by argentation thin-layer chromatography (TLC) and reversed phase TLC. They were identified as demospongic acids, 5,9-hexacosadienoic (26:2), 5,9,19-hex acosatrienoic (26:3), and 5,9,20-heptacosatrienoic acids (27:3) by gas chromatographic-mass spectro metry analysis of their picolinyl esters and GLC analysis of the oxidative ozonolysis products. The proportion of 5,9,20-27:3 (25.4%) in total fatty acids was much higher than that had been observed for other sponges. The contents of 5,9-26:2 (10.0%) and 5,9,19-26:3 (7.2%) were comparable to those reported. All three demospongic acids were abunduntly found in the lipid classes of phosphatidylser ines and phosphatidylethanolamines.

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This paper presents the positional distribution of very longchain fatty acids, 24:6(n-3), in triacyl-sn-glycerols (TG) of flathead flounder (Hippoglossoides dubius). Each of the liver and flesh TGs was subjected to the stereospecific analysis. The liver TGs contained 24:6(n-3) at concentrations of 1.5, 1.2 and 1.7 mole % in the sn-1, sn-2 and sn-3 positions, respectively, and the flesh TGs had 9.0, 7.8 and 7.1 mole % in the sn-1, sn-2 and sn-3 positions, respectively. This fatty acid was distributed almost evenly among the three positions of the TGs. No preference for the sn-2 position was observed in contrast to the general tendency for the distribution of longer-chain polyunsaturated fatty acids, such as 22:6(n-3), 22:5(n-3) and 20:5(n-3). There was essentially no difference in the positional distributions of the liver and flesh TGs. The results obtained in this study give new fundamental information to the investigation of very long-chain fatty acids.

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