Background: Wound healing is a complex biological process. Fibroblast is one of cell which is responsible for the wound healing process. Virgin Coconut Oil (VCO) have health benefit as antibacterial, antiinflamatory, antioxidant, and immunomodulatory. This research was purposed to know the influence use of VCO over the amount of fibroblast in wound healing after dental extraction.Method: Research quasi experimental with post test control group design and using 15 Rattus novergicus which were divided into three groups (control positive, VCO topical application, and VCO per oral application). Tooth removal was committed by using explorer, scalpel-blade, and arterial clamp. Then VCO applied everyday for 7 days. Day 7th Rattus novergicus were died, the socket after extraction were made into histological form, then the amount of fibroblast were counted with 5 field of views and made in average. Result: The number of fibroblast of the most numerous was VCO per oral ( 48,6 ± 49,8 ), VCO topical ( 36,2 ± 38,8 ), and control positive ( 33,4 ± 35,2 ). The results of the Saphiro Wilk test and Levene test acquired data normally distributed and homogeny, so it continued to One Way Anova and data showed there were significant difference among the groups of samples with value p<0,05.Conclusion: The conclusion of this research is VCO could increase the number of fibroblasts in wound healing after dental extractions

ABSTRACT

Background: Extraction has been known as a method for removing the tooth from its socket. Although extraction is frequent, the incidence of post extraction complication are commonly found, about 2,6-30,9%. Complications can inhibit wound healing and bone regeneration of socket. PRP is Platelet Rich Plasma containing growth factors that plays an important role in wound healing, induces bone regeneration and stimulates osteoprogenitor cell into osteoblast cell. Chitosan is a chitin derivate that extracted from the crab shells through deacetylation stage. Chitosan properties are osteoinductive, biocompatible, biodegradable, cell proliferation, antimicrobial, antioxidant, antitumor, and stimulates growth factors capabilities. Purpose: To analyze the combination of PRP and Chitosan gel to increase the number of osteoblast in post extraction wound of wistar rat teeth.Method: True experimental post-test only control group design was done in 28 wistar rats. Sample were divided into 4 groups: PRP gel, combination PRP and Chitosan gel, Chitosan gel and control group Povidone Iodine. Dextra Mandible incisors were extracted and treated accordingly. Number of osteoblast in post extraction socket was observed microscopically after 14 days using IHC staining. Data analyzed by using One Way ANOVA parametric test followed by Post Hoc LSD test.Results: There were statically signifcant di?erence between number of osteoblast in combination PRP and Chitosan gel group compare to PRP gel group (p=0,736), Chitosan gel group (p=0,402) and Povidone Iodine group (p=0,613).Conclusion: Combination PRP and Chitosan gel has a good e?ect in increasingnumber of osteoblast in wound healing post dental extraction

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Background: Child anxiety is a worries that comes from unknown source and most important factor that caused the problems of child visits to check the condition of oral health to dentist. Music applied as distraction to relieve anxiety, music can provide comfort and relaxation that becomes way to lowering psychological anxiety. Purpose of this study is to determine effect of instrumental music of Kitaro and traditional music of Javanese Langgam on the level of child's anxiety before dental treatment. Method: Quasi-Experimental research with pretest-posttest design contol group was conducted on 74 samples were divided into 2 groups: instrumental music of Kitaro and traditional music of Javanese Langgam. Anxiety levels were measured by Wong Baker Face Scale questionnaire and pulse. Hypothesis was tested by Wilcoxon test on Wong Baker Face Scale questionnaire and paired sample t-test on measurement of pulse. To determine child's anxiety level differences between two music were analyzed by Mann-Whitney test. Result: Wilcoxon test results on both music showed p=0.000 and results of paired sample t-test on both music showed p=0.000 this showed there were significant difference between before and after treatment. On Mann-Whitney test showed p=0.096 at Wong Baker Face Scale questionnaire and p=0.006 at pulse measurement, this showed there were significant difference between two groups. Conclusion: From these results concluded that there were difference effect of instrumental music of Kitaro and traditional music of Javanese Langgam in anxiety levels of children before dental treatment.