Sea buckthorn is one of the most important eco-economic tree species in China due to its ability to grow and produce acceptable yields under limited water and fertilizer availability. In this study, the differentially expressed genes under drought stress (DS) of sea buckthorn were identified and compared with control (CK) by RNA-Seq. A total of 122,803 unigenes were identified in sea buckthorn, and 70,025 unigenes significantly matched a sequence in at least one of the seven databases. A total of 24,060 (19.59%) unigenes can be assigned to 19 KEGG pathways, and 1,644 unigenes were differentially expressed between DS and CK, of which 519 unigenes were up-regulated and 1,125 unigenes down-regulated. Of the 47 significantly enriched GO terms, 14, 7 and 26 items were related to BP, CC and MF, respectively. KEGG enrichment analysis showed 398 DEGs involved in 97 different pathways, of which 119 DEGs were up-regulated and 279 DEGs were down-regulated under drought stress. In addition, we found 4438 transcriptor factors (TFs) in sea buckthorn, of which 100 were differentially expressed between DS and CK. These results lay a first foundation for further investigations of the very specific functions of these unigenes in sea buckthorn in response to drought stress.

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A novel CO2 sensor based on SERS with excellent selectivity and rapidity was prepared by coating ultrathin ZIF-8 on Au-dotted Ag-nanowires.

In order to further research the relationship between classical swine fever virus’ (CSFV) NS3 protein and the cytopathic effect (CPE) in cells infected with the CSFV, and to reveal the effect of protein NS3 on the host cells, the NS3 of CSFV Shimen strain amplified by RT-PCR was subcloned into the pEGFP-C1, named pEGFP-C1-NS3. The insert position, the size and the reading frame were correct for restriction enzyme digestion and sequence analysis. The pEGFP-C1-NS3 and pEGFP-C1 were transfected into PK-15 cells by liposome, and positive cell clones were gained by G418. The NS3-EGFP fusion protein expressed in pEGFP-C1-NS3 cells was observed by inverted fluorescence microscopy and identified by Western blot. The CPE appeared in positive pEGFP-C1-NS3 cells 72 h after passaging, apoptosis detection was also performed on positive pEGFP-C1-NS3 cells and pEGFP-C1 cells 72 h after passaging by TUNEL assay. The apoptosis rates in the positive pEGFP-C1-NS3 and pEGFP-C1 cells were 43.4 and 13.1%, respectively (p < 0.05). The results suggest that the CPE in positive pEGFP-C1-NS3 cells was induced by apoptosis and there is a relationship between the expression of NS3 and apoptosis.

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