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2015) The changes and relationship of structure and functional properties of rabbit myosin during heat-induced gelation,The objective of this study was to track the changes and the relationship between structure and functional properties of rabbit myosin during heat-induced gelation, and to provide more insight into the gelation properties of rabbit myosin. Storage modulus (G′) and loss modulus (G′′) during the heat-induced gelation process were measured by a rheometer, and the water holding capacity (WHC) and hardness of the heatinduced gel were measured by centrifugation method and texture analyzer, respectively. The quantitative analysis of secondary structural content was performed by circular dichroism (CD). The CD data were fitted to three secondary structures, which were α-helix, β-sheet, and random coil. The results showed that the WHC, hardness, G′, G′′, β-sheet, and random coil increased with the increase of temperature, while the α-helix decreased. The changes of structural properties of myosin determined the functional properties during the heat-induced gelation process, and they had a very strong correlation.El objetivo de este estudio consistió en dar seguimiento a los cambios ocurridos durante la gelificación inducida por calor y a la relación existente entre la estructura y las propiedades funcionales de la miosina de conejo durante dicho proceso. Asimismo, el estudio pretendió generar más información acerca de las propiedades de gelificación de la miosina de conejo. En el transcurso del proceso de gelificación inducida por calor, se midieron el módulo de almacenamiento (G′) y el módulo de pérdida (G′′) mediante el uso del reómetro; mientras que, la capacidad de retención de agua (WHC) y la dureza del gel inducida por el calor, fueron medidas empleando el método de centrifugación y el analizador de textura, respectivamente. El análisis cuantitativo del contenido estructural secundario fue realizado mediante dicroísmo circular (CD). Los datos obtenidos a partir del CD se aplicaron a tres estructuras secundarias: hélice α, lámina β, y espiral al azar (random coil). Los resultados demostraron que cuando se incrementó la temperatura, la WHC, la dureza, G′, G′′, la lámina β y la espiral al azar aumentaron, mientras que la hélice α disminuyó. Los cambios experimentados por las propiedades estructurales de la miosina determinaron las propiedades funcionales durante el proceso de gelificación inducida por calor, demostrando, además, un grado de correlación muy alto.

Background: Salvia miltiorrhiza is a traditional Chinese medicine with remarkable antioxidant, antibacterial, and anticoagulant properties. In the present study, we investigated the effects of Salvia miltiorrhiza injection in protecting Schwann cells (SCs) from hydrogen peroxide (H 2 O 2 )-induced cell apoptosis.Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence staining were used to detect the establishment of the SC apoptosis model induced by H 2 O 2 . The effect of Salvia miltiorrhiza injection on injured cell morphology was observed, and the effect on cell apoptosis was determined by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Western blotting analysis was used to detect the effect of Salvia miltiorrhiza injection on apoptosis-related protein expression. Results:The results of the MTT assay showed that cell activity significantly decreased after treatment with 1 mM H 2 O 2 , but different concentrations of Salvia miltiorrhiza injection could improve cell activity at different degrees. The number of cells increased significantly after treatment with Salvia miltiorrhiza injection. Annexin V-FITC/PI double staining and TUNEL results revealed that Salvia miltiorrhiza injection could significantly reduce apoptosis induced by H 2 O 2 . Western blotting analysis showed that the expression of Bcl-2 was significantly upregulated, while the expression level of Bax was significantly downregulated.Conclusions: Salvia miltiorrhiza injection can protect SCs from H 2 O 2 -induced cell apoptosis, and has potential therapeutic effects in neurological disease.

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