The aim of this study is to compare the effects of ethacridine lactate and hypochlorous acid on wound healing in rats through clinical and histopathological studies. The rats were divided into three groups; group 1; control group, group 2; hypochlorous acid (HOCL) group, group 3; ethacridine lactate (EL) group. Each group contained seven animals. Under anesthesia, a 20 mm long full layer skin resection was performed from dorsal interscapular region. Wound sizes were measured with millimetric paper on the 3rd, 7th and 14th day postoperatively. At the end of the 14th day, the animals were sacrificed under deep anesthesia and extensive skin resection of the wound area was performed and sent for histopathological examination. Macroscopic examination of wounds revealed that the wound was completely closed without any crust formation in the HOCL group, and also there was only a scar left in some animals of the HOCL group at the end of 14th day. Mild inflammatory cell, intense fibroblast activity and the lowest SOD and GPx immunoreactivity were found in the HOCL group compared to the other two groups (P<0.05). Consequently, it was observed that macroscopically and histopathologically, the wound healing was faster in animals treated with HOCL compared to those who were in the EL and the control group.