Membrane-free stem cell components (MFSCC) from basal adipose tissue-derived stem cells (ADSCs) are unknown for the treatment strategies in osteoarthritis (OA). OA has been considered to be associated with inflammatory damage and cartilage degradation. In this study, we intended to investigate the molecular mechanism of the anti-inflammation and cartilage protection effect of MFSCC in vitro (rat primary chondrocytes) and in vivo (rat OA model). The MFSCC treatment significantly inhibited interleukin-1α (IL-1α) stimulated inflammation and cartilage degradation. The MFSCC considerably reduced the levels of inflammatory factors such as iNOS, COX-2, NO, and PGE2 and was suppressed NF-κB and MAPKs signaling pathways in IL-1α-stimulated rat chondrocytes. Additionally, biomarkers of OA such as MMP-9, COMP, and CTX-II decreased in the monosodium iodoacetate (MIA)-induced rat OA model by MFSCC treatment. In conclusion, the MFSCC was established to suppress IL-1α induced inflammation and cartilage degradation in vitro and in vivo. These findings provide new insight for understanding OA therapy using membrane-free stem cell approaches.

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Background Pectolinarigenin (PEC) is one of the flavonoid active ingredients, and the anti-inflammatory and anti-cancer effects of PEC are being studied continuously. In a previous study, we found out the PEC regulates PI3K/AKT/mTOR pathway on gastric cancer (GC) cells. The purpose of this study was to establish the GC regulatory function of PEC in vivo and in vitro. Methods In this research, PEC was intraperitoneally administered to confirm tumor control after xenografting the AGS cell line into BALB/c nude mice to form tumors. Subsequently, tumors were extracted and LC-MS analysis and Gene ontology were performed for proteomics. Results The body weight and hematological analysis showed that PEC was not toxic to the non-cancerous cells in the xenograft mice model. We identified 582 proteins related to cellular responses such as tumorigenesis and cell death siganling in the tumor tissue. 6 out of 582 proteins was regulation similar by PEC in vivo and in vitro. Conclusion Our findings indicated that PEC treatment might suppress tumor growth in GC, and proteomic analysis provides the basic information about proteins that could be of significant potential as novel therapeutic targets againsg GC.

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