Temu hitam (Curcuma aeruginosa Roxb.) is a member of Zingiberaceae (ginger) family with health benefits includes increasing appetite, cleansing after childbirth, and curing coughing with phlegm, dispnea, and skin diseases. Temu hitam is commonly consumed in the form of instant jamu preparation. However, lack of quality control of herbal drugs production leads to many frauds in producing the jamu temu hitam. Fingerprint chromatography using TLC video densitometry can provide information on integrity, similarities, and differences of chemical components in herbal extracts or products quickly and easily. The purpose of this study was detecting adulterant (turmeric) qualitatively in jamu temu hitam using fingerprint analysis by TLC video densitometry. The fingerprint TLC patterns were recorded after separation using silica gel GF254 nm as stationary phase, and mixture of n-hexane: ethyl acetate (3:1.5, v/v) as mobile phase. Spot detection and recording were conducted using mirrorless camera after derivatization with anisaldehyde reagent and under UV lights at 254 nm and 365 nm. The spot was transformed by TLC analyzer software. Chemometric method with principal component analysis (PCA) was used to produce score and loading. The grouping data of both turmeric and temu hitam was used to analyze adulterant in instant jamu products. Of the three tested: jamu temu hitam products, two products contained no turmeric adulterant because it had the same characteristics as those of temu hitam and one product did not contain both temu hitam as well as turmeric. The last product was suspected to contain other adulterants. The profile of fingerprint from TLC video densitometry could be used to differentiate the characteristics of temu hitam and turmeric and hence was useful for spotting adulterants in jamu temu hitam.