Sabun cuci piring dan sabun mandi merupakan perbekalan kesehatan rumah tangga (PKRT) yang hampir pasti dibutuhkan oleh setiap rumah tangga. Pada sebagian rumah tangga, pengeluaran untuk kedua jenis produk tersebut cukup memberatkan secara ekonomi. Produksi sabun mandi dan sabun cuci piring yang relatif sederhana prosesnya dengan bahan-bahan yang relatif mudah didapat, diharapkan berdampak positif terhadap perekonomian mereka. Kegiatan ini dilakukan dilingkungan masyarakat Babakan Baru, kelurahan Sukapada, kecamatan Cibeunying Kidul, Kota Bandung. Kegiatan terdiri dari ; pretest, penyuluhan tentang sabun mandi dan sabun cuci piring, produksi dan pengemasan sabun cuci piring dan sabun mandi, dan diakhiri dengan posttest. Masyarakat peserta umumnya menyatakan senang dan merasa terbantu dengan adanya kegiatan ini.
Timbal merupakan logam berat yang dihasilkan dari limbah industri dan ditemukan dalam perairan. Timbal dalam jumlah kecil tidak berbahaya bagi manusia namun jika jumlahnya melampaui batas dapat menyebabkan keracunan akut maupun kronis. Tujuan penelitian ini adalah untuk mengetahui kandungan logam berat timbal dalam kerang hijau, kerang bulu, dan sedimen di perairan Teluk Jakarta. Metode yang digunakan adalah Spektrofotometri Serapan Atom, meliputi validasi metode analisis yang terdiri atas penetapan linieritas, batas deteksi dan batas kuantitasi, akurasi dan presisi, serta analisis kadar timbal pada sampel. Hasil penelitian menunjukkan nilai linieritas 0,9971, batas deteksi 0,04 µg/g, batas kuantitasi 0,14 µg/g, akurasi untuk sampel kerang dan sedimen berturut-turut adalah 99,66% dan 87,64%, nilai koefeisen variasi untuk sampel kerang dan sedimen berturut-turut adalah 0,64% dan 1,07%. Hasil analisis kadar timbal dalam kerang hijau adalah 13,98±1,924 µg/g, kerang bulu adalah 33,64±4,66 µg/g, dan sedimen adalah 28,6720±1,06 µg/g. Hasil analisis kadar timbal dalam sampel menunjukkan bahwa kandungan timbal berada di atas ambang batas yang ditetapkan oleh Badan Pengawasan Obat dan Makanan No.HK.00.06.1.52.4011 yaitu 1,5 µg/g.
Food allergies are one of the most common allergies in Indonesian society. Generally, when children aged 5-6 years food allergies will disappear, except peanut allergies and allergies to seafood, such as fish, shellfish and crustaceans. This study aims to determine the pattern of separation of allergen proteins in shrimp using anion exchange column chromatography method and identify allergen proteins in shrimp using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. Protein extraction from shrimp using Phosphate buffer saline (PBS) pH 7.2 and centrifuged at 10,000 rpm for 10 min at 4�C. Protein separation was carried out by anion-exchange column chromatography method, and the fraction obtained was measured at 280nm wavelength. The highest yield at absorbance was identified by using SDS-PAGE. Polyacrylamide gel electrophoresis was used to determine the protein profile and molecular weight of shrimp extract. Coloring of protein bands using silver staining. Data were analyzed descriptively based on the migration value of the sample protein bands compared to the marker protein band (Rf). The results of protein allergen profile analysis on shrimp using SDS-PAGE showed that the shrimp contained a protein band with a molecular weight of 37.77 kDa for cooked shrimp and 37.03 kDa for fresh shrimp.
Temu hitam (Curcuma aeruginosa Roxb.) is a member of Zingiberaceae (ginger) family with health benefits includes increasing appetite, cleansing after childbirth, and curing coughing with phlegm, dispnea, and skin diseases. Temu hitam is commonly consumed in the form of instant jamu preparation. However, lack of quality control of herbal drugs production leads to many frauds in producing the jamu temu hitam. Fingerprint chromatography using TLC video densitometry can provide information on integrity, similarities, and differences of chemical components in herbal extracts or products quickly and easily. The purpose of this study was detecting adulterant (turmeric) qualitatively in jamu temu hitam using fingerprint analysis by TLC video densitometry. The fingerprint TLC patterns were recorded after separation using silica gel GF254 nm as stationary phase, and mixture of n-hexane: ethyl acetate (3:1.5, v/v) as mobile phase. Spot detection and recording were conducted using mirrorless camera after derivatization with anisaldehyde reagent and under UV lights at 254 nm and 365 nm. The spot was transformed by TLC analyzer software. Chemometric method with principal component analysis (PCA) was used to produce score and loading. The grouping data of both turmeric and temu hitam was used to analyze adulterant in instant jamu products. Of the three tested: jamu temu hitam products, two products contained no turmeric adulterant because it had the same characteristics as those of temu hitam and one product did not contain both temu hitam as well as turmeric. The last product was suspected to contain other adulterants. The profile of fingerprint from TLC video densitometry could be used to differentiate the characteristics of temu hitam and turmeric and hence was useful for spotting adulterants in jamu temu hitam.
ABSTRAKFosfor merupakan nutrisi penting bagi manusia dan hewan. Selain itu, fosfor juga penting untuk pertumbuhan, pemeliharaan, perbaikan semua jaringan tubuh, dan dibutuhkan bersama dengan kalsium dan magnesium untuk pertumbuhan dan pembentukan tulang pada bayi dan anak-anak. Penelitian ini bertujuan untuk mengetahui kandungan fosfor dalam kubis putih dan ungu. Fosfor diukur dengan metode spektrofotometri Visibel, meliputi validasi analisis yang terdiri atas penetapan linieritas, batas deteksi dan batas kuantisasi, akurasi dan presisi serta analisis kadar fosfor pada sampel. Hasil penelitian menunjukan nilai linieritas 0,9991, batas deteksi dan kuantisasi dihitung secara statistik, dengan nilai 0,38 µg /ml dan 1,25 µg / ml. Presisi intra-day dan inter-day Nilaiinyatakan sebagai koefisien variansi (CV) ditentukan dalam tiga hari yang berbeda. Nilai presisi inter-day masing masing 0,67; 0.60; dan 0,80%, untuk presisi intra-day 0,82% . Perolehan kembali tingkat konsentrasi 2,0; 3,0; 4.0; dan 5,0 ug / ml kisaran 86,33% -105,01%. Kadar fosfor dalam kubis putih 20,725 mg/g, dalam kubis ungu 14,525 mg/g Dari hasil tersebut kadar fosfor dalam kubis putih ternyata lebih besar dibandingkan dengan kubis ungu tetapi kadar Fosfor tidak dipengaruhi oleh warna kubis. Kubis merupakan makanan kaya serat dan dapat dijadikan sebagai bahan makanan alternatif sebagai sumber fosfor.Kata Kunci : Fosfor (p), spektrofotometri, kubis. ABSTRACTPhosphorus is an essential nutrient for human and animal. In addition, phosphorus is also important for the growth, maintenance, repair of all body tissues, and is required along with calcium and magnesium for growth and bone formation in infants and children. The purpose of this study was to determine the levels of phosphorus in the cabbage white and purple. The methode used spectrophotometry visible,which conttain validation includes linierity determination, the limit of detection and limit of quantitation, accuracy and precision, and analysis of phosphorus in the sample. The results showed that linearity r value of 0.9991 the value of the limit of detection of 0,38 µg/mL and quantitation limit value of 1.25 µg/mL, the precision inter day: 0.67%; 0.60%; and 0.80%. and also for the intra day: 0.82%. Value of percent recovery were 101.76 -105.01%. The phosphorous content of white cabbage 19,344 mg/g and 22,116 mg/g, the purple cabbage 16,458 mg/g and 12,591 mg/g. From these results cabbage white phosphorous content was bigger than the purple cabbage, but levels of Phosphorus is not affected by the color of the cabbage. Cabbage is a food rich in fiber and can be used as an alternative to high food ingredients containing phosphorus.
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