Mosquitoes as one of the most common but important vectors have the potential to transmit or acquire a lot of viruses through biting, however viral flora in mosquitoes and its impact on mosquito-borne disease transmission has not been well investigated and evaluated. In this study, the metagenomic techniquehas been successfully employed in analyzing the abundance and diversity of viral community in three mosquito samples from Hubei, China. Among 92,304 reads produced through a run with 454 GS FLX system, 39% have high similarities with viral sequences belonging to identified bacterial, fungal, animal, plant and insect viruses, and 0.02% were classed into unidentified viral sequences, demonstrating high abundance and diversity of viruses in mosquitoes. Furthermore, two novel viruses in subfamily Densovirinae and family Dicistroviridae were identified, and six torque tenosus virus1 in family Anelloviridae, three porcine parvoviruses in subfamily Parvovirinae and a Culex tritaeniorhynchus rhabdovirus in Family Rhabdoviridae were preliminarily characterized. The viral metagenomic analysis offered us a deep insight into the viral population of mosquito which played an important role in viral initiative or passive transmission and evolution during the process.

Wuhan city is the biggest city in central China and has suffered subsidence problems in recent years because of its rapid urban construction. However, longtime and wide range monitoring of land subsidence is lacking. The causes of subsidence also require further study, such as natural conditions and human activities. We use small baseline subset (SBAS) interferometric synthetic aperture radar (InSAR) method and high-resolution RADARSAT-2 images acquired between 2015 and 2018 to derive subsidence. The SBAS-InSAR results are validated by 56 leveling benchmarks where two readings of elevation were recorded. Two natural factors (carbonate rock and soft soils) and three human factors (groundwater exploitation, subway excavation and urban construction) are investigated for their relationships with land subsidence. Results show that four major areas of subsidence are detected and the subsidence rate varies from −51.56 to 27.80 millimeters per year (mm/yr) with an average of −0.03 mm/yr. More than 83.81% of persistent scattered (PS) points obtain a standard deviation of less than −6 mm/yr, and the difference between SBAS-InSAR method and leveling data is less than 5 mm/yr. Thus, we conclude that SBAS-InSAR method with Radarsat-2 data is reliable for longtime monitoring of land subsidence covering a large area in Wuhan city. In addition, land subsidence is caused by a combination of natural conditions and human activities. Natural conditions provide a basis for subsidence and make subsidence possible. Human activities are driving factors and make subsidence happen. Moreover, subsidence information could be used in disaster prevention, urban planning, and hydrological modeling.

Relative sea level rise at tide gauge Galveston Pier 21, Texas, is the combination of absolute sea level rise and land subsidence. We estimate subsidence rates of 3.53 mm/a during 1909–1937, 6.08 mm/a during 1937–1983, and 3.51 mm/a since 1983. Subsidence attributed to aquifer-system compaction accompanying groundwater extraction contributed as much as 85% of the 0.7 m relative sea level rise since 1909, and an additional 1.9 m is projected by 2100, with contributions from land subsidence declining from 30 to 10% over the projection interval. We estimate a uniform absolute sea level rise rate of 1.10 mm ± 0.19/a in the Gulf of Mexico during 1909–1992 and its acceleration of 0.270 mm/a2 at Galveston Pier 21 since 1992. This acceleration is 87% of the value for the highest scenario of global mean sea level rise. Results indicate that evaluating this extreme scenario would be valid for resource-management and flood-hazard-mitigation strategies for coastal communities in the Gulf of Mexico, especially those affected by subsidence.

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