Summary of main observation and conclusion In this work, a new simple and readily synthesized turn‐on probe 2‐(4‐anthracene‐9‐yl‐phenyl)‐ 2H‐[1,2,3]triazole‐4‐carbaldehyde (APTC) was legitimately designed towards homocysteine (Hcy). Moreover, APTC has excellent optical properties such as intramolecular charge transfer (ICT) and aggregation induced emission enhancement (AIEE) characteristics, indicating its extensive application potentiality. What's more, APTC displayed rapid, high selectivity and specificity towards homocysteine over cysteine/glutathione. The detection limit of APTC for Hcy was as low as 2.198×10–8 mol·L–1, and the response time was only 5 min. APTC has been successfully applied to detect Hcy in silica gel plates and living cells, which indicates that APTC has good stability and biocompatibility as a selective probe for Hcy. Finally, the mechanism was studied using 1H NMR titration experiments and mass spectrometry.

A new fluorescence probe 2-(4′-(diphenylamino)-[1,1-biphenyl]-4-yl)-2H-[1,2,3]-triazole-4-carbaldehyde (DBTC) was designed and synthesized through Suzuki coupling reaction between (4-(diphenylamino)phenyl)boronic acid and (4-bromophenyl)-2H-[1,2,3]-triazole-4-carbaldehyde. This probe DBTC had intramolecular charge transfer and aggregation-induced emission enhancement performances and showed high selectivity and sensitivity toward homocysteine (Hcy) in the presence of other amino acids. The detection limit of probe DBTC for Hcy was 3.05 × 10 −6 M, and the mechanism was researched by 1 H NMR titration experiments and mass spectrometry. Furthermore, cell-culture results indicated that probe DBTC was cell permeable and could be used to detect Hcy in living cells. The good characteristics of the probe DBTC had huge application potential use for researching the effects of Hcy in other biological systems.

Synthesis and Optical Properties of Novel Spiro[chromo(2,3-c)pyrazole-4,1'-isobenzofuran]-3'-one Compounds

). 在二甲基亚砜(DMSO)/H 2 O (V∶V＝7∶3, pH＝7)溶液体系中, S1 对 Hg 2＋ 展示出 快速响应、高选择和高荧光灵敏性. 通过 1 H NMR 和 HRMS 探究了可能的机理. 聚丙烯酰胺(PAM)掺杂 S1 (PAMS)对 Hg 2＋ 有高的吸附性能, 其去除率达到 99.99%, 并且在紫外灯下通过肉眼很容易区分. 通过扫描电子显微镜(SEM)观察 了 PAMS 吸附前后的微观形貌. 此外, S1 具有优异的生物相容性, 可以极好地检测 HeLa 细胞中的 Hg 2＋ .

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