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Chloroplasts are organelles that contain genetic materials (DNA) in higher plant cells. The special genetic characteristics of chloroplasts mean that plasmid transformation has important research value, so it has become an important research direction second to nuclear transformation. Although the techniques of chloroplast genome modification have been successfully applied in tobacco and extended to other high plants, there are still many limitations. Exogenous genes are integrated into the chloroplast genome through homologous recombination. Therefore, the low efficiency of homologous recombination directly limits transformation efficiency. Gene editing with fixed-point cutting function and DNA damage repair mechanism may effectively improve the efficiency. In the present study, we aimed to use CRISPR/Cas9 to cut the site between two homologous recombinant fragments in chloroplast transformation to improve the efficiency by activating the DNA damage repair mechanism. The Cas9 gene and gRNA were added to the chloroplast transformation system of tobacco by co-transformation or integration into a transformation vector. The acquired resistant plants were screened by multiple selection of spectinomycin and chloroplast DNA was isolated for molecular detection by PCR. The results showed that the efficiency of chloroplast transformation increased by 6–10 times with the addition of gene editing technology. Although the transformation efficiency was still far below the level of nuclear transformation, this study may help to increase the efficiency of the plant chloroplast transformation system, and expand the types of plant receptors.

A new regional scale SEEBASE® model has been produced for the intracratonic Canning Basin, located in the north of Western Australia. The 2017 Canning Basin SEEBASE model is more than an order of magnitude higher resolution than the 2005 OZ SEEBASE version — the average resolution is ~1 : 1 M scale with higher resolution in areas of shallow basement with 2D seismic coverage — such as the Broome Platform and Barbwire Terrace. Post-2005 acquisition of potential field, seismic and well data in the Canning Basin by the Geological Survey of Western Australia (GSWA), Geoscience Australia and industry provided an excellent opportunity to upgrade the SEEBASE depth-to-basement model in 2017. The SEEBASE methodology focuses on a regional understanding of basement, using potential field data to interpret basement terranes, depth-to-basement (SEEBASE), regional structural geology and basement composition. The project involved extensive potential field processing and enhancement and compilation of a wide range of datasets. Integrated interpretation of the potential field data with seismic and well analysis has proven quite powerful and illustrates the strong basement control on the extent and location of basin elements. The project has reassessed the structural evolution of the basin, identified and mapped major structures and produced fault-event maps for key tectonic events. In addition, interpretative maps of basement terranes, depth-to-Moho, basement thickness, basement composition and total sediment thickness have been used to calculate a basin-wide map of basement-derived heat flow. The 2017 Canning Basin SEEBASE is the first public update of the widely used 2005 OZ SEEBASE. All the data and interpretations are available from the GSWA as a report and integrated ArcGIS project, which together provide an excellent summary of the key features within the Canning Basin that will aid hydrocarbon and mineral explorers in the region.

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