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Polypyrrole filmwas obtained by Chronopotentiometry method of electrochemical polymerization, with paratoluenesulfonic acid as supporting electrolyte and triple-electrode as working system. The polypyrrole film was characterized by infrared spectra (IR) and Scan electron microscope (SEM); And how its electrical conductivity affected by polymerization temperature and pyrrole concentration was studied. A glucose oxidase electrode with polypyrrole film as immobilizing carrier was prepared by a multiple-step electrochemical polymerization. The effects of reaction conditions such as temperature, solution pH, glucose concentration on the electrode bioelectrochemical response were discussed. Experimental results showed that the linear response range of glucose oxidase electrode was 1.0×10 -6 2.0×10 -2 mol/L and the detection limit was 1.0 10 -7 mol/L. Its response time was 9 seconds.

Copper-mediated graft-copolymerization of methyl methacrylate (MMA) onto casein was studied in this paper. The casein-g-PMMA was isolated by solvent extraction and was identified by Fourier transform infrared spectra (FTIR). Transmission electron microscope (TEM) micrographs revealed the presence of core-shell particles where PMMA was the core and casein was the shell. A glucose oxidase electrode with immobilizing carriers of casein-g-PMMA and polypyrrole membrane was prepared by a multiple-step electrochemically polymerization. The effects of reaction conditions such as working voltage, glucose concentration, temperature and solution pH on the characteristics of electrode bioelectrochemical response were discussed. The sensitivity and stability of the electrode were also investigated. Experimental results showed that the multiple enzyme electrode's linear range was 1.0×10 -6 1.43×10 -2 mol/L and the detection limit was 1.0 10 -7 mol/L the stable response time was 8 seconds. The electrode exhibited favorable repeatability and selectability, and its lifetime was greater than one month. The glucose biosensor will have a widest application in clinical diagnosis.