Background: Ovarian carcinoma (OC) is one of the most common malignancies of the female reproductive organs, with a low survival rate primarily due to the lack of effective methods for early diagnosis and prognosis. Objective: In this article, our motivation is to explore the lncRNA-related networks mechanisms involvement in the pathogenesis of OC. Methods: Public lncRNAs and mRNA expression datasets for OC were collected from the Gene Expression Omnibus (GEO) database. By integrated bioinformatics analysis, we constructed a UCA1-miRNA-mRNA network. We studied lncRNA-related molecular modulation mechanism in ovarian cancer cells based on MTT assay, dual luciferase reporter gene assays, quantitative real-time PCR, and western blotting. Results: UCA1 was higher in ovarian tumor tissues and cells than normal tissues and cells. It was demonstrated in this study that knockdown of UCA1 inhibited ovarian cancer cell viability which a miR-99b-3p inhibitor could reverse in vitro. Further, UCA1 was shown to regulate the expression of SRPK1 by directly binding to miR-99b-3p. Conclusions: These results suggested that UCA1 functioned as an oncogene in ovarian cancer. Inhibition of UCA1/ miR-99b-3p/SRPK1 axis may become a novel target for treating ovarian cancer.

MicroRNAs (miRNAs/miRs) have important roles in inflammation and infections, which are common manifestations of acute respiratory distress syndrome (ARDS). The present study aimed to assess whether serum miRNAs are potential diagnostic biomarkers for human ARDS. For this, two sets of serum samples from healthy individuals and patients with ARDS were analysed by high-throughput sequencing to identify differentially expressed genes in ARDS. A total of 679 valid sequences were identified as differentially expressed (P<0.05). Of these, five differentially expressed miRNAs were subjected to reverse transcription-quantitative PCR validation. Finally, two miRNAs (miR-584 and miR-146a) were successfully verified. These two miRNAs were significantly downregulated in the serum of patients with ARDS. Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that their target transcripts were implicated in a broad range of biological processes and various metabolic pathways, including involvement in the regulation of various inflammatory factors. The present study provided a framework for understanding the molecular mechanisms of ARDS and suggested that miR-584 and miR-146a are associated with ARDS and may be potential therapeutic targets.

In this study we investigated anticancer effect of Moringa oleifera leaf extract on A2780 ovarian cancer cell line. The leave of Moringa oleifera were grinded into powder and the phytochemicals were extracted using 80% ethanol. For fractionation and partitioning process four kinds of solvents were used in order: Petroleum ether, Dichloromethane, Ethyl acetate and Water extract until the extract is colorless or lightened. For cell proliferation assay all samples were dissolved in DimethylSulfoxide. Anticancer effect of Moringa oleifera were tested on A2780 ovarian cell line with four different extracts using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. The final result showed that on Petroleum Ether, Dichloromethane and Ethyl Acetate extracts as the concentration increases from 1 µg/ml, 2 µg/ml, 3 µg/ml, 4 µg/ml inhibited A2780, despite that in water extracts when the concentration decreases the inhibitory effect increases, however, Dichloromethane Extract has higher anticancer effect on A2780 ovarian cancer cell line with insignificant cytotoxicity to the normal cell.