No abstract

The objective of this study was to determine the effects of mercury chloride (HgCl) on laying performance, egg quality, hepatic and renal histopathology, and serum biochemical profiles in laying hens. A total of 768 Hy-line brown laying hens aged 40 weeks were randomly allocated to four groups (8 pens per group and 24 hens per pen). The concentrations of mercury (Hg) in four groups were 0.280, 3.325, 9.415, and 27.240 mg/kg. Results revealed that dietary Hg could significantly reduce laying performance (P < 0.05) and egg quality (P < 0.05) and was dose-dependently deposited in albumen, yolk, eggshell, and whole egg. Meanwhile, the thicknesses of palisade layer, mammillary layer, and total layer, and the percent of palisade layer were significantly decreased (P < 0.05), while the percent of mammillary layer was sharply increased (P < 0.05) in eggshell. In addition, with increasing dietary dosage of Hg, accumulation of Hg in viscera was significantly increased (P < 0.05), and histopathological damages in liver and kidney were more and more severe. Serum alanine aminotransferase, aspartate aminotransferase, and globulin were significantly increased (P < 0.05), while serum albumin and albumin to globulin ratio were significantly decreased (P < 0.05) in 27.240 mg/kg Hg group. Blood urea nitrogen, uric acid, and creatinine were significantly increased (P < 0.05) in 3.325, 9.415, and 27.240 mg/kg Hg groups. These results suggested that dietary HgCl could reduce laying performance and egg quality with hepatic and renal function disorders in laying hens.

Propolis has been shown to reduce the level of blood glucose and suppress the histopathological changes in diabetics. However, it still remains unknown if propolis has a similar effect on diabetic retinopathy (DR). Our study aimed to evaluate the effect of the ethanol extract of Chinese propolis (EECP) on early DR in streptozotocin (STZ)-induced diabetic rats. EECP was given to diabetic rats by oral intubation for 12 weeks. The concentrations of fasting blood glucose (FBG), glycated hemoglobin (HbA1c), malondialdehyde (MDA), reactive oxygen species (ROS), and reactive nitrogen species (RNS) were measured. Pathological examinations, including hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), and immunofluorescence, were also conducted to provide further evidence of EECP's effect on early DR. EECP was able to attenuate diabetes via directly decreasing the levels of FBG and HbA1c, which also resulted in the reduction of MDA, ROS, and RNS. Furthermore, EECP could protect against the damages of photoreceptor cells, as well as retinal thickening. And the inhibition of blood-retinal barrier (BRB) leakage was also observed in EECP-treated diabetic rats, along with the inhibition the loss of tight junction proteins (occludin, ZO-1). These results suggest that EECP has an ameliorating effect on early DR by inhibition of blood-retinal barrier breakdown.Practical Application: This study sheds light on the protective effect of the ethanol extract of Chinese propolis on early diabetic retinopathy and the molecular actions underlying the inhibition of blood-retinal barrier breakdown. Our study suggests that ethanol extract of Chinese propolis can be considered as a potential therapeutic agent in the treatment of early diabetic retinopathy.

No abstract

No abstract