The aim of this study was to evaluate the effect of fluoride-releasing adhesive systems on human decalcified dentin in vitro. Two fluoride-releasing adhesive systems, Reactmer bond (RB, Shofu) and ABF (AF, Kuraray), an experimental system, and a commercial adhesive system without fluoride release, SE bond (SE, Kuraray), were used in this study. The amount of fluoride release from adhesive in deionized water was measured every week for 10 weeks. Class V cavities were prepared on extracted human pre-molars and decalcified dentin was promoted by using a bacterial caries induction system at the cavity floor. The cavities preserving decalcified dentin were restored with resin composite (AP-X, Kuraray) after treatment by each adhesive system. The specimens without treatment by adhesive system and restoration were used for control. The specimens with restoration were then incubated for 4 weeks at 37 degrees C, 100% humidity. Microradiographs of the specimens showed that the radiopacities of the decalcified dentin layers in RB and AF groups with fluoride release were significantly higher than those in SE or control groups without fluoride release. This result suggested that the fluoride-releasing adhesive systems enhanced mineralization of decalcified dentin.

No abstract

(3A3173).We investigated the distribution of heat shock protein 47 (hsp47) in cultured chicken embryonic chondrocytes and epiphyseal chondrocytes of tibial bones from 1-day-old to 6-week-old chickens. Northern blot and immunoblot analyses revealed that hsp47 exists in epiphyseal cartilage and cultured chondrocytes. Confocal laser immunofluorescence microscopy showed that hsp47 was localized mainly in the many granular structures found in the cytoplasm that contain Type 11 collagen. Epiphyseal cartilage and cultured chondrocytes were embedded in LR White resin and hsp47 was detected by protein A-immunogold electron microscopy.

The aim of this study was to evaluate the fluoride release, neutralizing ability and inhibitory effect on secondary caries of resin-based materials containing a silane-coated glass filler. Resin-based materials containing fluoro-boro-alumino-silicate glass coated by 3-methacryloxypropyltrimethoxysilane or 3-aminopropyltrimethoxysilane were used and resin material containing glass filler without coating was used as a control. The fluoride release and pH value after immersion were measured for 10 weeks. The inhibitory effect was also evaluated. During the initial period, the material with 3-aminopropyltrimethoxysilane gave the greater amount of fluoride release and produced a higher pH value compared with the other materials. However, the neutralizing ability of the material with 3-aminopropyltrimethoxysilane filler became weaker with ageing of the specimens. The mean depth of outer lesions was similar among the three materials. Within the limitations of this study, secondary caries around restorations could not be inhibited even for products showing high fluoride releasing and neutralizing ability.

Diastereomeric geminate pairs of chiral bis(2-oxazoline) ruthenium complexes with bipyridyl-type N-heteroaromatics, Λ- and Δ-[Ru(L-L)(2)(iPr-biox)](2+) (iPr-biox=(4S,4'S)-4,4'-diisopropyl-2,2'-bis(2-oxazoline); L-L=2,2'-bipyridyl (bpy) for 1Λ and 1Δ, 4,4'-dimethyl-2,2'-bipyridyl (dmbpy) for 2Λ and 2Δ, and 1,10-phenanthroline (phen) for 3Λ and 3Δ), were separated as BF(4) and PF(6) salts and were subjected to the comparative studies of their stereochemical and photochemical characterization. DFT calculations of 1Λ and 1Δ electronic configurations for the lowest triplet excited state revealed that their MO-149 (HOMO) and MO-150 (lower SOMO) characters are interchanged between them and that the phosphorescence-emissive states are an admixture of a Ru-to-biox charge-transfer state and an intraligand excited state within the iPr-biox. Furthermore, photoluminescence properties of the two Λ,Δ-diastereomeric series are discussed with reference to [Ru(bpy)(3)](2+).