Biofabrication of copper oxide nanoparticles (CuO-NPs) of a desired size remains a significant challenge. In this report, CuO-NPs were fabricated by treating 10 mM copper nitrate with Andean blackberry fruit (ABF) and leaf (ABL); and evaluated its antioxidant activity. As-prepared NPs characterization were determined by UVvisible spectrophotometry, Dynamic light scattering (DLS), Transmission electron microscopy (TEM) with selected area electron diffraction (SAED) and X-Ray Diffraction (XRD) analysis. UV-visible spectroscopy showed an electronic excitonic transition at 250 to 255 nm clearly reveals the formation of ABF and ABL CuO-NPs. DLS analysis demonstrated mean diameter of ABF CuO-NPs (43.3 nm) smaller than ABL CuO-NPs (52.5 nm). TEM with SAED confirmed the CuO-NPs are spherical and partial crystalline nature. Furthermore, the antioxidant efficacy of ABF CuO-NPs showed (89.02%, 1mM) whereas ABL CuO-NPs (75.92 %, 1mM) against 1, 1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that green CuONPs could be used effectively in future biomedical concerns.
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