Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type Ⅰ IFN production.Methods: To evaluate inhibitory effect of LJ on type Ⅰ IFN, we examined type Ⅰ IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type Ⅰ IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot.Results: LJ inhibited Type Ⅰ IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type Ⅰ IFN and IRF-1, 7 than LPS-induced LJ pretreatment.Conclusions: LJ inhibits LPS-induced production of type Ⅰ IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.