Concurrent reactivation of latent EBV and HAV infections is common. EBV reactivation with HAV infection adversely affects the clinical feature of hepatitis. Therefore, we should keep in mind that the concurrence especially in older children may cause worse injury to the liver.

Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type Ⅰ IFN production.Methods: To evaluate inhibitory effect of LJ on type Ⅰ IFN, we examined type Ⅰ IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type Ⅰ IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot.Results: LJ inhibited Type Ⅰ IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type Ⅰ IFN and IRF-1, 7 than LPS-induced LJ pretreatment.Conclusions: LJ inhibits LPS-induced production of type Ⅰ IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.