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Endocytosis is a multistep process involving the sequential recruitment and action of numerous proteins. This process can be divided into two phases: an early phase, in which sites of endocytosis are formed, and a late phase in which clathrin-coated vesicles are formed and internalized into the cytosol, but how these phases link to each other remains unclear. In this study, we demonstrate that anchoring the yeast Eps15-like protein Pan1p to the peroxisome triggers most of the events occurring during the late phase at the peroxisome. At this ectopic location, Pan1p recruits most proteins that function in the late phases—including actin nucleation promoting factors—and then initiates actin polymerization. Pan1p also recruited Prk1 kinase and actin depolymerizing factors, thereby triggering disassembly immediately after actin assembly and inducing dissociation of endocytic proteins from the peroxisome. These observations suggest that Pan1p is a key regulator for initiating, processing, and completing the late phase of endocytosis.

Synthesis of Enol Carbonates from a Reaction of SilylEnolates with 1H-Imidazolylurethane.-Treatment of silyl enolates (II) or (IV) with 1-alkoxycarbonyl-1H-imidazoles (I) provides a good method for the preparation of enol carbonates (III) and (V). The reaction proceeds presumably via an imidazolium intermediate. -(OHTA, S.; YAMASHITA, M.; NAGAI, N.; KAWASAKI, I.; MAEDA, K.; MIYANO, Y.; Heterocycles 41 (1995) 8, 1683-1689; Pharm. Univ., Yamashinaku, Kyoto 607, Japan; EN)