This study was performed to obtain information on the basic reproductive ecology and biological resource management of Buccinum osagawai. Samples were collected from August 2018 to October 2019 with a drum-shaped net at a depth of 150–250 m along the continental shelf in Jumunjin, on the east coast of Korea. The species showed gonochorism and a lack of external sexual dimorphism. The overall sex ratio (F:M) was 1:1.2 (n = 549, F = 250, M = 299); as the size of the individuals increased, the proportion of males tended to decrease. The development pattern of the oocyte was synchronous, in which a dominant group of oocytes was identified within the same ovary. The size of the ripe oocyte was 82.3 (±22.6) × 125.5 (±22.0) μm. The spermatocyte development pattern was group-synchronous, in which multiple stages of germ cell populations were simultaneously identified within the same spermatogenic acinus. The gonad index (GI) for both males and females showed the highest value during June, after which it decreased sharply, and after August it was below 2.0. The main spawning season was from June to July, and the GI and stages of gonadal development did not show a pattern of seasonal changes. The main gonadal active season was from May to July, and both male and female gonadal development and maturation took place over a short period, whereas the recovery period after spawning was longer. At least 60.5% of the group, considering both males and females, showed maturity at 50.1 mm SH or more. Furthermore, the size of 50% group maturity was shown at approximately 50.0 mm SH.
This study was conducted to find out biological response of the oyster Crassostrea gigas exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.20, 0.40 and 0.80 mg/L). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and degeneration of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 15%-55%. Histological analysis of organ system illustrated expansion of hemolymph sinus, degeneration of epithelial layer and connective tissue layer of the mantle. Also, epithelial degeneration and disappearance of cilia band are recognized in the gill filament and it was observed disruption of epithelial layer and degeneration of basophilic cell and epithelial cell in the digestive tubules.
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