Anther culture method has commonly been performed two-step culture of callus induction during the plant regeneration stage. Two-step culture was generally required to a much longer duration of culture for regeneration. There are still needed to be solved the disadvantage of the two-step culture technique. Anther of F1 plants derived from Drimi2 *3 /AR1 of new plant type and improved rice cultivars were compared with method efficiency using one-step and two-step culture method. The results showed that one-step culture was more effective than two-step culture. Callus induction averaged 8.2% for one-step culture and 6.1% for two-step culture. The proportion of plants that survived after colchicine treatment was similar as 17.5% for one-step culture and 16.7% for two-step culture. However, there was a highly significant difference when results of plant regeneration were compared. Frequency of regenerated plant averaged 13.6% for one-step culture, which was 7.1% higher than two-step culture (6.5%). These results provide valuable data that may be used to establish an efficient plant regeneration system for rice cultures.

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