Demonstrated is possibility to apply "cross-flow" ultrafiltration method for O antigen of Vibrio cholerae M-41 Ogawa concentrating from germ-free centrifugate. Technological process of concentrating was optimized. Worked out were the regimes of conservation and cleaning of the ultrafiltration device. The prospects of "cross-flow" ultrafiltration method introduction in technology of cholera chemical bivalent vaccine production were determined.

Worked out is the experimental technology for protective antigens of Vibrio cholerae 569 B Inaba (cholera-anatoxin and O-antigen) concentrating by means of tangential ultrafiltration. Optimization of concentrating technological process is carried out. This technique makes it possible to obtain cholera vaccine components meeting all regulatory requirements.

Presented is mathematical model of kinetics of the process of O-antigen and cholera toxin synthesis during periodical submerged cultivation of V. cholerae 569В Inaba with limitation as regards carbonic substrate. The proposed model is based upon analysis of experimental data on V. cholerae 569В Inaba biomass and antigens accumulation, rate of growth and antigens release, and glucose utilization. Using Mathcad 15.0 software calculated are coefficients of differential equations entering into the mathematical model. Comparison of predicted and experimental data demonstrates that relative error of determination of concentrations of the synthesized substances, glucose and cholera vibrio is between 5 and 20 %. The proposed model permits to determine maximum output of final products and specify the parameters of cultivation process performance at different initial conditions.

The article describes the experience of personal teaching experience in the area of Biology, in the system in the conditions of full-time and distance learning. The authors considered the definitions of the concepts of "distance learning", developed by domestic researchers. The article showsthe essence of distance education, its state at the present level.The traditional and innovative approaches used by the staff of the department in the process of teaching Biology are described.The basic questions of teaching Biology are answered in the process of reading lectures, organizing recitations, organizing competitions and scientific works. A new aspect of the work of teachers is the use of distance learning. Analyzed by the results of the use session of distance learning Biology students. various aspects of the use of distance learning for future doctors are noted.The advantages and disadvantages of this knowledge system are discussed, the results of application are analyzing.

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