The purpose of the study is to establish the effect of different levels of physical activity on the psychophysiological characteristics of teenagers, serving as the basis for the design of a health-saving educational development path. In the course of the study, 175 teenagers (12-14 years old), including 63 girls and 112 boys (34 boys playing hockey), were examined. For all subjects, we evaluated the indicators capturing a simple and complex visual-motor reaction using the BioMouse-Research hardware-software complex. The obtained results characterize the optimal level of cerebral homeostasis, reflected in the average sensorimotor response of all examination groups falling within the age norm range. The authors argue that the developed motor skills of teenagers involved in sports contribute to a greater cerebral speed of processing elementary sensory information, as well as high activity and mobility of the nervous processes of the central nervous system in comparison to peers not involved in sports.