Background: With the steadily increasing trend of functional cosmetics consumption, the public is increasingly expecting the efficacy of cosmetics, and in order to meet these consumer needs, the development of various effective raw materials, as well as products through technical convergence research, has been activating focusing on dermatology. In the Republic of Korea, much attention was paid to cosmetic raw materials using human stem cells as a new growth engine at the end of the 2000s. Accordingly, the Ministry of Food and Drug Safety implemented the Regulations on Cosmetic Safety Standards, etc., which contains the safety standards of humanderived cell and tissue culture as raw materials for cosmetics. Methods: Twice a day, morning and evening, for 28 days, 3 mL of the sample provided for each subject was applied onto the entire face using an airbrush. The subjects were divided into a control group (C), an experimental group 1 (ADSC-CM 5% group, E1) and an experimental group 2 (polymersome-containing of ADSC-CM 5% group, E2), and there were 20 subjects in each group. Results: Moisture content on the U-zone, melanin and erythema of lid-cheek, overall size and depth of eye wrinkles, and skin roughness of the items showed significant differences among all three groups. More specifically, E1 had a higher rate of skin improvement than C, and E2 had the highest skin improvement rate in all items: skin moisture content, transepidermal water loss (TEWL), melanin and erythema of lid-cheek, eye wrinkles, and skin roughness of lid-cheek. Conclusions: The effectiveness of ADSC-CM was verified in all items: increases in moisture content, decrease in TEWL, and improvements of whitening and wrinkles. Moreover, the effectiveness of ADSC-CM was observed as higher in samples of polymersome-containing ADSC-CM than those of raw material ADSC-CM. Furthermore, percutaneous absorption was improved compared with previous studies on formulations in relation to polymersome at the same concentration of ADSC-CM, thereby resulting in higher effectiveness.

Background: The development of the beauty industry has been accelerated by the combination of the basic desire of humans to look beautiful and the trend of the age in which the down-aging phenomenon is caused by an aging society. Previously, therapy, which has been provided by experts, has now expanded and changed its category into everyday home care. Thus, skin care devices became common and widespread. Also, public attention has been focused on functional cosmetics such as whitening and wrinkling improvements of a home care product. However, the results of validation of the effectiveness of functional components and the use of skin care devices are rare. Methods: Middle-aged women were asked to apply 3 mL of ampule containing niacinamide 2% and adenosine 0. 04% to the face twice a day, once in the morning and the other in the evening, for 4 weeks. The control group (C) was asked to apply using hands while the experimental group (E) was asked to apply using airbrush. Results: The moisture content of the entire face has increased in all clusters, resulting from a combination of the moisture content of the niacinamide and the increase in the collagen content of the adenosine. L* was increased in all groups, but only E showed significant results. a* was increased in C and showed a significant decrease in E only. b* decreased in group C and increased in group E. The overall size of the wrinkles, the depth of the wrinkles, and the width of the wrinkles showed significant improvements in all groups, with higher rates of improvement in E. Conclusions: This study produced verification results on whitening effects of niacinamide, wrinkling improvement of adenosine, and the usefulness of air brushes. Previously, dermal absorption studies on soluble ingredients of cosmetics were limited to iontophoresis, while various methods of study design on the dispensing and physical effects of fine particles were expected. It also recommends a research design approach that is practical for home care, rather than approach using a professional program on the usefulness of skin care device.