The investigation of the unpasteurized milk and lactic cheese as for the species of the Enterococcus bacteria genus has been made as well as the determination of their sensitivity to anti–infective drugs. It has been established that mainly three types of enterococci have escaped of the unpasteurized milk and lactic cheese: E. faecalis, E. faecium and E. durans. Thus the main part of enterococci of the unpasteurized milk and lactic cheese has composed a kind of E. faecalis 53.4 ± 4.22 and 73.4 ± 6.71% respectively. The quantity of E. faecium escaped of the unpasteurized milk has been 34.7 ± 2.15%, that is 2.86 times more in accordance with their content in the lactic cheese, and the genus E. durans ranged from 5.3 ± 0.47 to 9.3 ± 0.74%. The sensitivity to anti–infective drugs in E. faecalis escaped of the lactic cheese has been significantly lower compared to E. faecalis strains escaped of the unpasteurized milk. Yes, such anti–infective drugs that have been almost 100% active to E. faecalis escaped of milk as vancomycin, furamag, amoxicillin have shown lower efficiency to E. faecalis of the lactic cheese, the sensitivity ranged from 97,2 to 82,6%. The sensitivity of E. faecalis of the lactic cheese to other anti–infective drugs that have been taken into the experiment has been 1,3 – 37,0 times (p ≤ 0,05) lower compared to E. faecalis of the unpasteurized milk.

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Investigate the antimicrobial properties of various concentrations of vegetable essential oils in combination with an oil solution of chlorophyllipt as possible ingredients for ointments for wounds. Microbiological tests were performed according to standard methods using E. coli test cultures, S. aureus, Str. agalactiae and P. fluorescens. The results of studies of antibacterial activity of both individual ingredients and their combinations are presented, namely: 4 % essential oil of Siberian pine, 2 % essential oil of eucalyptus, 1.5 % essential oil of cloves, 1.5 % of essential oil of cedar, 2.0 % tea tree essential oil and 1.0 % chlorophyllipt oil solution. Bacteria, which are usually typical agents of wound infections, have been found to be quite sensitive to the drugs tested. High activity of essential oils and oily solution of chlorophyllipt with respect to E. coli and S. aureus was revealed. In particular, a 1.0 % oily solution of chlorophyllipt caused staphylococcal growth retardation zones whose diameters were 1.4 times larger than the antibiotic clindamycin. It was determined that representatives of gram-negative microflora were more sensitive to the investigated essential oils and chlorophyll. The optimal composition of the experimental drug called “Ointment for wounds” is offered. The results of preclinical testing showed a sufficiently high efficiency compared to traditional means. The results obtained with regard to antimicrobial activity indicate the prospect of using preparations based on the essential oil of Siberian pine, eucalyptus, carnation, cedar, tea tree and oil solution of chlorophyllipt for the treatment of skin diseases in animals. This data will help to develop new effective and safe veterinary treatments for wound care.