In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter and root-specific Mll promoter. The human interferon alpha-2b gene was correctly translated in carrot plants according to Western blot analysis. The recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus replication in established piglet testicular cells. The results demonstrated the higher activity of interferon accumulated in carrot plants for young leaves (up to 50.7 × 10(3) IU/g FW) compared to the mature ones probably due to the degradation-susceptible nature of this protein. The taproot-expressing system could have also provided the sufficient protein amounts (up to 16.5 × 10(3) IU/g FW) and could possibly be used for generating interferon alpha-2b protein in planta for preventing and curing infectious diseases.

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This short communication a i m s a t elucidating the effect of high-temperature (HT) annealing on the phonon-phonon relaxation in transmutationally-doped (TD) n-Si crystals. The relaxation is known to govern not only the value but also the anisotropy of the heat e. m. f. caused by the drag of electrons by phonons of longitudinal (1) and transverse (t) polarization.In our study we used TD c r y s t a l s of n-Si which, apart from the common technological annealing (T = 800 OC, t = 2 h), were exposed to HT annealing (T = 1200 OC, t = 2 h) followed by cooling a t a r a t e of about 1 K/min. Below the crystals investigated before and after HT annealing will be denoted by the symbols 'orig.' and 'HT I , respectively.-/6/ P. I. 3ARANSKX1, V. V. SAVYAK, and L. A. SHCHERBINA, Fiz. Tekh. poluprov. -14, 302 (1980).

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