The Toll-like receptors (TLRs) play a pivotal role in innate immunity for the detection of highly conserved, pathogen-expressed molecules. Previously, we demonstrated that lipopolysaccharide (LPS, TLR4 ligand)-increased macrophage motility required the participation of Src and FAK, which was inducible nitric oxide synthase (iNOS)-dependent. To investigate whether this iNOS/Src/FAK pathway is a general mechanism for macrophages to mobilize in response to engagement of TLRs other than TLR4, peptidoglycan (PGN, TLR2 ligand), polyinosinic-polycytidylic acid (polyI:C, TLR3 ligand) and CpG-oligodeoxynucleotides (CpG, TLR9 ligand) were used to treat macrophages in this study. Like LPS stimulation, simultaneous increase of cell motility and Src (but not Fgr, Hck, and Lyn) was detected in RAW264.7, peritoneal macrophages, and bone marrow-derived macrophages exposed to PGN, polyI:C and CpG. Attenuation of Src suppressed PGN-, polyI:C-, and CpG-elicited movement and the level of FAK Pi-Tyr861, which could be reversed by the reintroduction of siRNA-resistant Src. Besides, knockdown of FAK reduced the mobility of macrophages stimulated with anyone of these TLR ligands. Remarkably, PGN-, polyI:C-, and CpG-induced Src expression, FAK Pi-Tyr861, and cell mobility were inhibited in macrophages devoid of iNOS, indicating the importance of iNOS. These findings corroborate that iNOS/Src/FAK axis occupies a central role in macrophage locomotion in response to engagement of TLRs.

This paper analyzed characteristics of different energy consumption quotas development method focusing on building of green campus as our sustainable development demand and pointed out the development of energy consumption quotas by colleges and universities based on building area per student per stage by use of regression analysis, gaved out the corresponding ratings ,and proposed that correction coefficient should be developed based on properties, scientific research ability and scientific research funds of colleges and universities.

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The paper gives energy consumption indexes of government organization office buildings at the current stage, and then analyses energy-saving potential of office buildings. Further, it discusses building consumption has a correlation between the heating form of air conditioning and personnel density. Finally, the paper presents the energy consumption quota and the formulating method of using energy quota of state organ office buildings at the current stage.

Established a hierarchy structure model and made a quantitative assessment of seven air-conditioning systems which are gas boiler plus electric refrigerating system、air-cooled heat pump、variable refrigerant volume air conditioning、ground-source heat pump air conditioning、centralized heat-supply plus electric refrigerating、gas direct-fired machine、energy storage on relevant professionality and indoor environment,initial investment,operating costs,operation and management,environmental impact,energy consumption.Finally we obtained an optimal scheme,which agreed with that in practice.