Analyzovat souvislost mezi podáním reversinu a zvýšenou plasticitou buněk DFAT schopných dělení na různé typy buněk. Metoda: Vykultivované buňky DFAT byly rozděleny do čtyř skupin podle dávky reversinu: na kontrolní (bez reversinu) skupinu a na skupiny s aplikací reversinu v dávkách 10 nM, 20 nM a 40 nM. Každá skupina prochází několika stadii vývoje před další diferenciací na kardiomyocyty (identifi kované expresí cTnT), buňky hladké svaloviny (vascular smooth muscle cells, VSMC) (označené expresí afta-SMA) a buňky cévního endotelu (identifi kované expresí CD31). Výsledek: V každé skupině buněk DFAT s aplikací reversinu byly nalezeny statisticky významné rozdíly v expresi cTnT, alfa-SMA a CD31 (p = 0,003; resp. < 0,001 a < 0,001). Post hoc analýza s použitím Tukeyova testu prokázala, že pouze ve skupině s reversinem v dávce 10 nM došlo ke statisticky významnému rozdílu oproti kontrolní skupině (p = 0,002) v expresi cTnT a ve skupinách s reversinem v dávkách 10 nM a 20 nM k rozdílu v expresi alfa-SMA a CD31 (p = 0,028, resp. p < 0,001). Závěry: Tato studie prokázala vztah mezi dávkou reversinu a zvýšenou plasticitou buněk DFAT schopných diferenciace na kardiomyocyty (cTnT), VSMC (alfa-SMA) a buňky cévního endotelu (CD31).

Background: There is an essential need for cardiomyocyte regeneration among patients with heart failure. Transplantation of dedifferentiated fat (DFAT) cells may lead to an improvement of cardiomyocyte regeneration among heart failure patients. We believe that DFAT cells are promising candidate cell sources for cardiac regeneration. However, the pathway underlying how DFAT cells of the adipose lineage differentiate into mature cardiomyocytes isn’t fully understood. Methods: We conducted an experimental laboratory study on isolated DFAT cells from adipose tissue of healthy adults. Then, we treated cells with different concentrations of reversine (10, 20 and 40 nM), and performed RNA extraction and cDNA synthesis. Next, we used a ceiling culture method based on the buoyancy properties of mature lipid-filled adipocytes. Stemness expression (Octamer-binding transcription factor 4 [Oct4], brachyury, Fetal liver kinase 1 [Flk-1]) was quantified by reverse transcription-quantitative (RT-q)PCR, while cardiomyocyte expression (Transcription factor GATA-4 [GATA4] and cardiac troponin T [cTnT]) was quantified by immunocytochemistry. Results: ANOVA with Tukey’s post-hoc found that 10 nM reversine increased greater Flk-1 expression compared to the control group (MD: 5.037 + 0.998; p < 0.001), but there were no significant changes among Oct4 (MD: 0.013 + 1.244; p = 0.99) and brachyury expression (MD: 0.157 + 0.084; p = 0.252). Kruskal-Wallis revealed that the expression of GATA4 (1.65 [0.41-1.98] to 0.015 [0.007-0.034]; p = 0.017) reduced significantly from day 7 until day 21 and cTnT (5.07 [6.62-8.91] to 8.22 [6.81-9.40]; p = 0.001) increased significantly from day 7 until day 21. Conclusions: Reversine could increase the expression of Flk-1, but it was unable to stimulate the expression of Oct4 and brachyury related to stem cell-ness. An optimal concentration of 10 nM reversine may have the greatest effect on enhancing the differentiation of DFAT cells into mature cardiomyocytes, as indicated by higher cTnT expression between cells.