No abstract

Solanum ferox or acid aubergine plants is one type of vegetable used as flavoring in food. Solanum ferox contains terpenoids, steroids, flavonoids, alkaloids and phenolics. Solanum ferox has antipyretic, antirheumatic, anti-asthma, antiviral and as a drug for syphilis. The purpose of this study is to test the antibacterial activity of leaf methanol extract of Solanum ferox plant. Leaf Solanum ferox is extracted using methanol. Methanol extract was tested for antibacterial activity using diffusion method to use E.coli, S.aureus and B.subtilis bacteria. Amoxicillin is used as a positive standard while negative controls are solvents used to dissolve samples. Antibacterial activity of methanol and amoxicillin extract showed inhibitory zones of E.coli of 7,72-11,67 mm and 19.97 mm, inhibitory zone to bacterium B.subtilis only at 5.7 μg/mL concentration of 11,29 mm and amoxicillin 18, 51 mm.

Senyawa kalkon merupakan prekursor dari flavonoid yang penting sebagai antioksidan. Kalkon memiliki berbagai bioaktivitas seperti antikanker, antimikroba, antijamur, antitumor dan anti-inflmasi. Pada penelitian ini dilakukan sintesis senyawa (E)-1-(3’-metoksifenil)-3-(3-nitrofenil)prop-2en-1-on dengan metode iradiasi gelombang mikro dan menggunakan katalis basa KOH. Senyawa yang dihasilkan diuji kemurniannya dengan KLT, uji titik leleh dan analisis HPLC serta telah dikarakterisasi dengan spektroskopi UV, FTIR, 1H-NMR dan MS serta diuji toksisitasnya dengan metode Brine Shrimp Lethality Test (BSLT). Senyawa ini menunjukkan aktivitas toksisitas yang kurang baik dengan nilai LC50 >200 μg/mL.

No abstract

Microorganism is important producer of novel bioactive natural products, particularly in the field of drug discovery. Co-culture methods is one of powerful emerging tools for enhancing the chemical diversity of microorganisms. This research used the local culture collection of Penicillium sp. LBKURCC34, the fungus isolated from peat soil of primary forest at Giam Siak Kecil Bukit Batu (GSKBB) - Biosphere Reserve in Riau Province, to produce secondary metabolites secreted to their growth media that was cultivated by two different Gram of bacterial pathogen, Escherichia coli and Staphylococcus aureus. The 14 days fermentation was carried out and the media was extracted with ethyl acetate. The ethyl acetate crude extract was evaporated, then the concentrate dissolved in methanol. Antifungal, Candida albicans test was performed by the disc diffusion and the resazurin-based turbidimetric method. This study found that the crude extract of the co-culture with S. aureus could inhibit C. albicans growth, while that extract of the co-culture with E.coli could not do. The value of minimum inhibitory concentration (MIC) of the potential extract was less than the positive control, Ketoconazole. It only has potency as bacteriostatic extract.