Plants provide a rich resource of medicinal material for research and development of new medicine. To discover new compounds as Immunosuppressant from plants, we evaluated the immunosuppressive effect of different fractions and particularly one compound (Calceolarioside A) that were extracted from the leaves of Fraxinus Mandshurica Rupr. The fractions and the compound were tested on the ability to reduce Immunoglobulin E (IgE) secretion by human U266 multiple myeloma cells (U266 cells) and to reduce interleukin-2 (IL-2) secretion by mouse spleen cells. Our results showed that both the butanol extract fraction and the compound of Calceolarioside A inhibited the IgE and IL-2 production in U266 cells and mouse spleen cells respectively, and no cytotoxicity was observed within the effective dose range. These results suggest that Calceolarioside A could potentially serve as an immunosuppressant.

This study was to investigate the vitamin levels in the serum of children in the Northeast of China and some factors associated with the vitamin levels. The sera were obtained from 2096 normal children aged 1 month to 12 years in the Northeast of China. Vitamins A and E were detected using their sera by HPLC, and the level of vitamin D was detected by LC-MS. The 20 overweight children were chosen from the 7-8 age range and took additional vitamins studies for extra 3 months. The data were analyzed by IBM SPSS Statistics 21. The average levels of vitamins A, D, and E in sera samples from the 2096 children were 0.2715mg/L (95%CI, 0.2715-0.2791mg/L), 26.2848μg/L (95%CI, 25.7900-26.7786μg/L), and 8.6137mg/L (95% CI, 8.5077-8.7198mg/L), respectively. The percentages of vitamins A, D, and E deficiency were 19.61%, 47.47%, and 0.62%, respectively. For 20 children from the VDD or VAD groups, after giving them more VA and VD for 3 months, the levels of VA and VD in the sera were increased significantly; however, the average BMI has barely changed. According to our finding, vitamin D deficiency was severe, vitamin A deficiency was common, and vitamin E was insufficient in the sera of children in the Northeast of China. The levels of vitamins A, D, and E were correlated with age and overweight.

According to our finding, the levels of vitamin A, D, and E in the sera of pregnant women in northeastern China were affected by where they live and their age. Vitamin D deficiency was very serious, vitamin A deficiency was common, while vitamin E seems to be sufficient.

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