Submerged plants can create a suitable microenvironment for calcium and phosphorus co-precipitation (CaCO3-P) to further enhance the removal of phosphorus from the water column. To investigate the effect of calcium addition on the phosphorus removal in water bodies of submerged plants, this paper used Elodea nuttallii as a research object and monitord the phosphorus content in water column, sediment, plants and water environmental factors. The results showed that the addition of calcium was more effective in removing phosphorus than the group without calcium, and the final phosphorus content was controlled at a lower level; the effect of the calcium group on the phosphorus content of sediment was greater, and the addition of calcium promoted the increase of calcium-phosphorus content in the sediment. Moreover, the phosphorus content in the plant of the calcium group was higher than the plant group. It is clear that the addition of calcium ions contributed to both the removal of phosphorus from the water column and the enrichment of phosphorus by the plants.

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Using Biber's MD model, this paper investigates the register variation in crisis translation and discovers that learned exposition is the text type that comes closest to crisis translation. Crisis translation has “explicit” and “informational” features, according to statistics. When compared to the non-translational “learned and scientific” corpus, the translated corpus includes feature of “nominalization.” Furthermore, the researchers discovered that in Biber's MD model, the variables AWL and PHC had a strong liner connection with NOMZ.

e21003 Background: Pulmonary large cell neuroendocrine carcinoma (LCNCE) is a high-grade neuroendocrine tumor with poor prognosis. The genomic profile and immune feature of LCNEC have been reported in many studies. However, the relationship between immune microenvironment and the prognosis of LCNEC patients is poorly understood. Our study was aimed to examine the infiltration of CD8+ T cell and PD-L1+ cells in LCNEC patients, and explore whether CD8+T cell and PD-L1+ cells are related to LCNEC patients’ prognosis. Methods: 37 eligible LCNEC patients (stage I-III) with received the treatment of surgical operation (27 among 37 patients underwent adjuvant radiochemotherapy) were included, tumor tissues were collected from the first medical center of Chinese PLA General Hospital. Multiplex immunohistochemistry (Multi IHC) was performed using the antibodies of CD8 and PD-L1. The univariate and multivariate cox proportional hazards analyses were used to assess the association between the abundance of CD8+T cell and PD-L1+ cells with the clinical outcome of LCNEC patients. Results: Patients were divided into high and low group using the median positive percentage of CD8 or PD-L1 as cutoff. The univariate analysis showed that patients with stromal high CD8+ T cell infiltration or stage I had significantly longer disease free survival (DFS) (CD8, p = 0.03; stage I, p = 0.036), and patients with high PD-L1 expression in stroma region, I stage, adjvant radiochemotherapy had notably increased overall survival (OS) (p = 0.049, p = 0.031, p = 0.023, respectively). However, the infiltration of CD8+ T cell or PD-L1 expression in total or tumor region had no correlation with survival of LCNEC patients. Furthermore, multivariate analysis demonstrated that low CD8+ T cell density in stroma region was an independent risk factor for PFS (p = 0.03), advanced TNM stage (II-III stage, p = 0.0009) and low stromal PD-L1 expression were independent risk factors for OS (p = 0.013). Conclusions: High infiltration of CD8+ T cell or PD-L1+ cell in stroma region were independent good prognosis factors of LCNEC patients. However, these findings need a larger LCNEC cohort to validate.