Posterior capsular opacification (PCO), resulting from the undemanded intracapsular cell proliferation, is the most common complication of intraocular lens (IOL) implantation after cataract surgery. In recent years, IOL has become...

Posterior capsule opacification (PCO), the most common complication after cataract surgery, is caused by the proliferation, migration, and epithelial-mesenchymal transition (EMT) of residual lens epithelial cells (LECs) in the capsule bag. Although the surface modification and drug loading of intraocular lens (IOLs) have been effective in preventing PCO to some extent, the intraocular safety of anti-proliferative drug application is still a major limitation in clinical application. In this study, we used non-viral gene delivery systems in combination with layer-by-layer (LBL) self-assembly technology, and the modified IOL could effectively prevent the development of PCO by interfering with the EMT process mediated by the Platelet-derived growth factor receptor-α (PDGFR-α). Herein, the gene fragments were wrapped by electrostatic conjugation using polyethyleneimine-graft-poly(ethylene glycol) (PEI-g-PEG) to form gene complexes. Gene complexes were characterized by DLS, TEM and agarose gel electrophoresis, and evaluated for storage and serum stability. The layer assembly behavior of the IOL surface, changes in optical properties, and the release behavior of the gene complexes were characterized using quartz crystal microbalance (QCM), UV-vis, contact angle (CA), and TEM. In vitro experiments showed that the IOL coating has good bio-compatibility and can achieve the corresponding transfection effect, and the released gene complexes exhibited excellent cell internalization and lysosomal escape behaviors, as well as effective inhibition of PDGFR-α expression and its mediated EMT process. The early PCO prevention effect and bio-compatibility evaluation of the modified IOL in vivo were evaluated by implantation into animal eyes. This study provides a new strategy for the development of surface modifications of small nucleic acid drugs and non-toxic EMT interference therapies for PCO.

Correction for ‘Design of foldable, responsively drug-eluting polyacrylic intraocular lens bulk materials for prevention of postoperative complications’ by Yueze Hong et al., J. Mater. Chem. B, 2022, DOI: https://doi.org/10.1039/d2tb01974d.