Research of the frequency of increased ketone bodies in cows during the transition period three weeks before calving and three weeks after calving should determine the tendency of the Holstein breed to ketosis. It is also important to investigate the relationship between changes in blood biochemical parameters and the development and treatment of ketosis. The purpose of the study was to determine the effectiveness of using a probiotic strain of bacteria in subclinical ketosis in cows during drying off and after calving. The following methods were used: testing for determining the level of beta-ketones in the blood of cows; biochemical method of blood testing; clinical method; statistical method. A study of cows in the drying off group showed that the level of β-ketones ranged from 0.2 to 1.0 mmol/L. Application efficiency of Bacillus Pumilus L. A 56 in a concentration of 1×109 , CFU/g at a dose of 30 g per animal was determined to be 100%. In the study of metabolic changes in the body of cows after calving patients with ketosis, the content of total protein, albumins globulins of the enzyme aspartate aminotransferase, and urea at the beginning of the study was increased and went beyond the maximum permissible limits of the reference level. After seven days of using the probiotic, the content of ketone bodies and biochemical parameters decreased to normal. It was proved that at the end of the study, the activity of alanine aminotransferase increased, the level of potassium, vitamins A and E increased. Thus, it was determined that the enzyme alanine aminotransferase is one of the metabolites, an increase in the level of which indicates the accumulation of lipids in the liver. In addition, the content of Ca, P, and vitamin D decreased during treatment, which indicates the risk of hypocationemia. In the group of cows after calving, the incidence of ketosis was 27%. The therapeutic efficacy of a probiotic of 35 g per animal in the postcalving distribution group was 73%. The practical value of the study lies in the prevention of subclinical ketosis in cows in deep drying off and after calving, reducing the cost of treating concomitant diseases, and culling animals

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