CRISPR-Cas systems not only play key roles in prokaryotic acquired immunity, but can also be adapted as powerful genome editing tools. Understanding the native role of CRISPR-Cas systems in providing adaptive immunity can lead to new CRISPR-based technologies. Here, we develop CRISPRminer, a knowledge base and web server to comprehensively collect and investigate the knowledge of CRISPR-Cas systems and generate instructive annotations, including CRISPR arrays and Cas protein annotation, CRISPR-Cas system classification, self-targeting events detection, microbe–phage interaction inference, and anti-CRISPR annotation. CRISPRminer is user-friendly and freely available at http://www.microbiome-bigdata.com/CRISPRminer.

Phage-host interactions are appealing systems to study co-evolution. Their roles in human health and diseases as well as novel therapeutics development also have been increasingly emphasized. Meanwhile, such interactions leave signals in bacterial and phage genomic sequences, defined as phage-host interaction signals (PHIS), allowing us to predict novel phage-host interactions. Due to the intrinsic complexity and recent emerging of metagenomics sequencing data, there is an urgent requirement to develop computational tools to analyze massive data and extract meaningful information. Here, we seize comprehensive in silico PHIS and utilize sophisticated bioinformatics to develop PHISDetector, a web tool to detect and systematically study diverse in silico PHIS, including analyses for co-occurrence/co-abundance patterns, oligonucleotide profile/sequence composition, CRISPR-targeting, prophages, phage genome similarity, protein-protein interactions, and special gene check. PHISDetector accepts various genomic and metagenomic data as input and provides well-designed visualizations and detailed data tables to download. Prediction tasks are processed remotely by the server using custom python scripts and a series of public tools. PHISDetector can be accessed at

The “Guiding Opinions on the Comprehensive Utilization of Bulk Solid Waste during the 14th Five-Year Plan” issued by the Ministry of Ecology and Environment in 2021 pointed out that by 2021, China’s cumulative bulk solid waste stockpiles are about 60 billion tons, and the annual new stockpiles are nearly 3 billion. At the same time, there is a problem of low solid waste utilization rate, occupying a large amount of land resources, and a large hidden danger to the ecological environment. In order to further improve the utilization of solid waste, reduce the cost of road construction, and reduce the damage and impact of solid waste accumulation on the environment. This paper analyzes phosphogypsum impaction of the incorporation of various solid wastes on asphalt penetration, softening point, ductility and other properties, as well as the impact on the high temperature stability, low temperature crack resistance and water stability of asphalt mixtures, and the summarize and evaluate the microscopic modification mechanism and other aspects. At present, there is a relatively lack of research on the reuse of some solid wastes in asphalt in China. This review can provide reference and reference for judging a variety of solid wastes as asphalt modifiers, promote the green reuse of solid wastes, and reduce the construction of asphalt pavements.