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The purpose of this study was to develop Biology learning media with Mixed Reality applications using Pyramid Holograms. This research is a qualitative descriptive study. The research was conducted in two stages, namely the problem identification stage and the development stage. The problem identification stage is done by questionnaire and student test results and the development stage by designing Mixed Reality media. The results of the study were in the form of motion system material media with Pyramid Hologram technology that can be used in the learning process.

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Research to examine students’ motivation and learning activities through constructivism-based STEM learning has been carried out from January to April 2020 at “SMP Purnama Pekanbaru”. The design of one group used a purposive sampling technique, that is class IX which chooses extracurricular biotechnology. Data collection used observation sheets of learning activities and questionnaire sheets about student learning motivation. Data of frequency and percentage is calculated and analyzed descriptively.The results show that learning biotechnology through the constructivism-based STEM approach is effective in increasing motivation and recycling activities of waste into fish pellets.