No abstract

Violacein and deoxyviolacein display a broad range of interesting biological properties but their production is rarely distinguished due to the lack of suitable analytical methods. An HPLC method has been developed for the separation and quantification of violacein and deoxyviolacein and can determine the content of both molecules in microbial cultures. A comparison of different production microorganisms, including recombinant Escherichia coli and the natural producer Janthinobacterium lividum, revealed that the formation of violacein and deoxyviolacein is strain-specific but showed significant variation during growth although the ratio between the two compounds remained constant.

No abstract

No abstract

Optimization and modeling of cultivation processes for the production of heterologous and homologous proteins requires a comprehensive characterization of product formation. Methods have been developed for the experimental investigation of mass transport, substrate turnover and product formation in biopellets of the fungus Aspergillus niger for the production of glucoamylase (GAM). GAM production in A. niger was chosen as a model system, its advantage being its potential for complex, posttranslational protein modifications.