ABSTRACT. The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of polymorphic alleles per locus ranged from 2 to 11. The observed and expected heterozygosities were 0.050-0.913 and 0.049-0.869, respectively. The loci identified in this study could provide a useful tool for research on genetic diversity and genetic differentiation of A. pectinata populations.

ABSTRACT. The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish that inhabits the beach rocky bottom from Japan to the South China Sea. Eleven polymorphic microsatellite loci were developed from S. marmoratus and were used to identify polymorphisms in 30 samples from a wild population. The allele locus number ranged from 2 to 7. Polymorphism data content ranged from 0.032 to 0.751. The observed and expected heterozygosity levels were 0.0333-0.9667 and 0.0328-0.7675, respectively. Two loci, Smd1-112 and Smd2-80, deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers will facilitate further studies of genetic diversity and genetic differentiation of S. marmoratus.