Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)

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ABSTRACT. Lunella coronata granulata, from the family Turbinidae, is an economically important species. The first set of 10 polymorphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559. Eight loci were in Hardy-Weinberg equilibrium (HWE) (P > 0.05), while two loci deviated significantly from the HWE after Bonferroni's correction (P < 0.005). The isolated microsatellite loci can be utilized in studies of population genetic analysis and they provide important genetic markers for construction of genetic linkage maps and genetic breeding of L. coronata granulata resources.

Section: Introductionmentioning

confidence: 99%

Short Communication Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)

et al. 2015

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“…Therefore, the development of microsatellite markers can contribute significantly to obtaining more detailed insight into population genetics. Some microsatellite markers from A. pectinata have been isolated by Liu (2009) andChen (2012). However, more effective microsatellite loci will be beneficial to the study of population genetics and construction of a genome map.…”

Section: Introductionmentioning

confidence: 99%

Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)

Bai

Yang

et al. 2014

ABSTRACT. In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to 0.8954. Values for observed heterozygosity (H O ) and expected heterozygosity (H E ) ranged from 0.0714 to 0.9231 and from 0.1948 to 0.9237, respectively. Four loci deviated significantly from Hardy-Weinberg equilibrium. The newly developed microsatellite markers will be beneficial in assessing the genetic diversity, population structure and genetic conservation of A. pectinata, and in other relevant research.

“…Microsatellite markers, which are powerful co-dominant genetic markers, have been widely applied to studies of population genetics, linkage analysis, and resource conservation in various marine organisms, including Branchiostoma belcheri Gray (Dai et al, 2013), Fenneropenaeus penicillatus (Shangguan et al, 2014), pen shell (Chen et al, 2012), and other animals and plants (Fopp-Bayat and Ciereszko, 2012), but few reports of molecular markers are available in H. leucospilota. Microsatellites, also known as simple sequence repeats (SSRs) or short tandem repeats, are repeating sequences of 1-6 base pairs (bp) of DNA (Schlötterer and Pemberton, 1998).…”

Section: Introductionmentioning

confidence: 99%

Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota

Jb¹,

Zb²,

Yf³

et al. 2015

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ABSTRACT. The sea cucumber Holothuria leucospilota has high medicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individuals from a wild population. The polymorphic information content ranged from 0.183 to 0.668, and the number of alleles per locus varied from 3 to 5. The observed and expected heterozygosities were 0.0370-0.5000 and 0.0776-0.6250, respectively. With the exception of 3 loci (Y1-15, Y11-1, and Y28), the polymorphic loci were in Hardy-Weinberg equilibrium (P > 0.003125). These polymorphic microsatellite loci will contribute to studies of genetic diversity, the research of population structure, and the design of conservation strategies for H. leucospilota.