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The anthoeyanins of the genus Hibiscus (family Malvaceae) have not hitherto been studied. In this communication we give the results of an investigation of the anthocyans of hybrid forms of hibiscus. Numeroushybrid forms of hibiscus have been obtained by N. F. Rusanov by interspecies hybridization of the north American species of Hibiscus' H. moscheutos, H. militaris, and H. coccineus, which are widely represented in the Botanical Garden of AS UzSSR.The flowers of the hybrid hibiscus of the variety M. Gor'kii were extracted with methanol containing 1% of HCI. The anthoeyanins were precipitated from the extract with a threefold volume of ether. The total anthocyanins were separated by chromatography on a column of cellulose powder in the water-acetic acid-concentrated HCI (82 : 15 : 3) system. Two individual crystalline substances were obtained. After recrystallization from 2 N HCI in ethanol, the first anthoeyanin formed a dark violet microcrystalline powder with a golden tinge having mp 215-217 ° C (decomp.) with the composition C21H21OIICI. 2H20; UV absorption spectrum: kma x 525 m# [i].The second anthocyanin formed a red-brown microcrystalline powder with a golden tinge melting at 239-241 ° C (deeomp.) with the composition C26H29015C1 • 4H20; UV absorption spectrum: kma x 523 m#.The results of a study of the products of enzymatic and acid hydrolysis, oxidative degradation, and alkaline fusion, and UV spectroscopy and paper chromatography in the presence of markers enabled the first anthocyanin to be identified as chrysanthemin and the second as gossypicianin, which have previously been isolated from the flowers of the cotton plant [2].

Continuing a study of the anthoeyanins [1] of the family Malvaceae, by partition chromatography on a column of cellulose powder [in the water-acetic acid (85 : 15) system] we have isolated from the flowers of Hibiscus cannabinus var. simplex (kenaf) collected in the experimental section of the central Asian Branch of VIR ]All-Union Scientific Research Institute for Plant Breeding] an anthocyanin with mp 164-166 ° C (decomp.) having the composition C26H29016C 1.4H20. Hydrolysis with a 2 N solution of HC1 and with the enzyme of Aspergillus oryzae led to the decomposition of the anthocyanin into an aglycone, glucose, and xylose. On stepwise acid hydrolysis, paper chromatography showed the production of a monoglycoside of delphinidin.

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