The in vitro methods of Biotechnology have been used for analyzing of germination stages and grow conditions of Vaccinium L. subgenera spread in Armenia (Vaccinium myrtillus L. and Vaccinium uliginosum L.). These studies reveal that optimum conditions for in vitro germination of wild blueberry Vaccinium myrtillus and Vaccinium uliginosum seeds were: cold stratification 4-5 0C for 8 weeks, wherein the percentage of germinated seeds was 74.2 – 88.3%. The regenerative potential of shoot and root formation of the analyzed subgenera’s depends on the grow regulators composition of the medium. This Vaccinium L. in vitro study can be used for future using in breeding systems and production.