The aim of study to determine out the role of CoQ10 in reducing the side effects from the use of Tramadol on the level of Interlukin IL-6, IL-10 and PGF2α the study included 30 endemic female rabbits, where each group included  5 animals. The results showed increase with a highly significant with (P≤0.01) in the concentration of IL-10 in sera of treated group with tramadol (initial dose) and group treated tramadol (double dose) group treated tramadol (initial dose + CoQ10) and group treated  with tramadol (double dose + CoQ10) compare with control. The results showed no significant differences in the concentration of IL-10 in sera of treated group with Co Q10 compare with control. The results revealed  high significant increase  (P≤0.01) in the concentration of IL-6 in the sera of the treated group with  tramadol (double dose) group treated with  tramadol (initial dose + COQ10) as compared with the control and the results showed the presence of high significant decrease (P≤0.01) in the concentration of IL-6 in the sera of the group treated with tramadol (initial dose) and treated with group tramadol (double dose + COQ10) compare with control. the results showed there were no significant differences in the concentration of IL-6 in serum of group treated with CoQ10 as compare with control. Results indicated occurrence of high significant increase (P≤0.01) in a concentration of PGF 2α in serum of the group  treated with tramadol (initial dose) and group treated with tramadol (double dose)and group treated with tramadol (initial dose + CoQ10) and group treated with tramadol (double dose +CoQ10) compare with control. The results showed presence of a highly significant decrease (P≤0.01) in the concentration of P G F 2α in sera of group treated  with  CoQ10 as compare with control.