Pre-natal embryo sexing was done using circulatory blood of pregnant women at early stages of pregnancy, before the 4 th month of pregnancy, in which 20 samples were obtained the objective of the study was to establish and evaluate multiplex PCR for detection of Y-chromosome -specific sequence of fetal DNA in maternal blood circulation during pregnancy in which identification of fetal gender become possible before the 4 th month of pregnancy, this pre-test can be used to determine whether invasive prenatal diagnosis, such as amniocentesis and chorionic villi sampling should be performed on a fetus having a risk of X-linked recessive inheritance. The results of the study showed the appearance of a band with 57bp (represent the primer GAPDH) In all samples which represented the control. The second band with the 100bp (represent the primer Y1.7 and Y1.8) as well as the first band (57bp) appeared in male fetuses since it represent a gene found only on the Y chromosome. The application of a non-invasive method like multiplex PCR by using maternal blood for embryo sex identification reveals a reliable and accurate method for prenatal diagnosis.