Pests often found in homes and harmful to humans are mice (Mus musculus). In addition to most ap and destroyer of building, Mus musculus also acts as a spreader of disease for humans. Controls carried out by the community are making traps or traps, rat poison, and smoking. In addition, there is a natural way, namely using noni fruit. This study aims to determine the effectiveness of noni fruit extract (Morinda citrifolia) as a vegetable pesticide against the control of mice (Mus musculus) and to determine differences in the effectiveness of noni fruit extract (Morinda citrifolia) at each dose variation of 35%, 40%, 45%, 50 %, and 55%. This study uses a quasi-experimental design (quasi-experimental). The number of samples is 30 mice. Toxicity test on mice using the fixed-dose method. The statistical test used is the one-way Anova test. The results showed that there was an effect of the effectiveness of noni fruit extract (Morinda citrifolia) on the mortality of mice (p-value = 0.000 <0.05), and at a concentration of 55% it was effective for killing mice (Mus musculus). However, at a concentration of 55%, it cannot be said to be effective in killing mice (Mus musculus) because it can only kill 20% of mice.